Tissue Extracts and their Sensitivity to Factor VII and Factor X

Summary

Lung thromboplastins made by conventional methods are normally heavily contaminated with factor VII, due to interaction of tissue with plasma or serum, and calcium during their preparation. Factor X is consumed during this process, and the resulting thromboplastin is capable of detecting deficiency of factor X in a plasma sample.

Lung thromboplastin may be prepared free from factor VII contamination, and such thromboplastin is extremely sensitive in detecting factor VII deficiency.

• References

• 1 Biggs R, Macfarlane R. G. Human Blood Coagulation and Its Disorders. Blackwell Scientific Publications; Oxford: 1962
• 2 Denson K. W. The specific assay of Prower-Stuart Factor and Factor VII. Acta haemat 25: 105 1961;
• 3 Denson K. W. Levels of blood coagulation factors during anticoagulant therapy with Phenindione. Brit. med. J I: 1205 1961;
• 4 Montigel C. The mode of action of different thromboplastin preparations. Schweiz, med. Wschr 89: 1259 1959;
• 5 Straub W, Duckert F. The formation of the extrinsic prothrombin activator. Thrombos. Diathes. haemorrh. (Stuttg) 05: 402 1961;
• 6 Verstraete M, Clark P. A, Wright I. S. Evaluation of prothrombin time performed with different thromboplastins. Acta. med. scand 163: 105 1959;
• 7 Weiß H. J, Eikelberger J. W, Crosby W. H. Study on the differences in activity of lung and brain thromboplastin. 40: 205 1960;