Thromb Haemost 1964; 11(01): 075-084
DOI: 10.1055/s-0038-1654807
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

Preparation, Stabilization and Some Properties of Purified Human Plasmin*

Daniel L Kline
1   Department of Physiology, Yale University School of Medicine, New Haven, Connecticut
,
Jacob B Fishman
1   Department of Physiology, Yale University School of Medicine, New Haven, Connecticut
,
With the technical assistance of B. A. Lage› Author Affiliations

This work was supported by Grant HE-07162-01 from the United States Public Health Service and Grant GB 576, continuation of G-16107 of the National Science Foundation.
Further Information

Publication History

Publication Date:
24 July 2018 (online)

Preview

Summary

1. Lysine increased the solubility, decreased the SK requirement and increased the stability of plasmin prepared from purified plasminogen by SK activation.

2. A procedure is presented for the rapid and quantitative conversion of plasminogen to plasmin and storage of the plasmin in stable form at neutral pH as a lyophilized powder.

3. Approximately 10% for the plasminogen molecule was split off during its activation. No carbohydrate was lost.

4. The plasmin isolated was homogeneous in the ultracentrifuge at pH 2.5 and was quantitatively convertible to plasminogen activator by the addition of SK.