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Thromb Haemost 1971; 25(03): 545-554
DOI: 10.1055/s-0038-1654328
Originalarbeiten – Original Articles – Travaux Originaux
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The Evaluation of the Two-Stage Prothrombin Assay

H. C Hemker
1   Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
,
P. W Hemker
1   Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
,
K. v. d Torren
1   Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
,
P. P Devilee
1   Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
,
W. Th Hermens
1   Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
,
E. A Loeliger
1   Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands
› Author Affiliations
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Publication History

Publication Date:
28 June 2018 (online)

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Summary

It is shown that (and why) the traditional ways of evaluating a thrombin generation curve do not give acceptable estimations of the prothrombin content of the sample. An alternative method is shown to meet the requirements better; it consists of drawing the thrombin generation curve on a semi-logarithmic graph, and extrapolating the descending slope to zero time. This gives a thrombin concentration representing the prothrombin concentration of the original sample.

 

  • References

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