Effect of Aspirin in Vivo and in Vitro on Plasma Cofactors of Platelet Aggregation^[*]

Summary

It was found that suspension of normal washed platelets in a media which had been incubated with aspirin, or was derived from subjects taking aspirin, resulted in decreased platelet aggregation with ADP. The media consisted of either normal platelet poor plasma, fraction I-0 or heated plasma. When the latter two media were used, both the heated plasma and fraction I-0 were modified by pre-treatment of the donor subjects with aspirin or incubation of each fraction with aspirin, and this modification was a time-dependent reaction taking 5 to 10 min to take place. Furthermore, washed platelets exposed to aspirin and then washed and resuspended showed a decreased response to ADP. This was also a time-dependent reaction. It is concluded that aspirin affects not only the platelet but also the cofactors of ADP-induced platelet aggregation.

^* Supported by USPHS Grant #HE 10056.

• References

• 1 Al-Mondhiry H, Marcus A, Spaet T. H. Acetylation of human platelets by aspirin. Fed. Proc 28: 516 1969;
• 2 Aster R. H, Jandl J. H. Platelet sequestration in man. I. Methods. J. clin. Invest 43: 843 1964;
• 3 Beaumont J. L, Caen J, Bernard J. Action hémorrhagipare de l’acide acétyl-salicylique au cours des maladies du sang. Bull. Soc. Méd. Hôp. Paris 71: 1687 1955;
• 4 Beaumont J. L, Willie A, Lenègre J. Influence de l’acide acétylsalicylique sur l’hémostase. Bull. Soc. Méd. Hôp. Paris 71: 1077 1955;
• 5 Blatrix C. h. Allongment du temps de saignment sous influence de certains médicaments. Nouv. Rev. franç. Hémat 03: 346 1963;
• 6 Blombäck B, Blombäck M. Purification of human and bovine fibrinogen. Arkiv for Kemi 10: 423 1956;
• 7 Born G. V. R. Quantitative investigations into aggregation of blood platelets. J. Physiol 162: 67 1962;
• 8 Born G. V. R, Gross M. J. Effects of inorganic ions and of plasma proteins on the aggregation of blood platelets by adenosine diphosphate. Physiol 170: 397 1964;
• 9 O’Brien J. R. Effects of salicylates on human platelets. Lancet I: 779 1968;
• 10 Brinkhous K. M, Read M. S, Mason R. G. Plasma thrombocyte-agglutinating activity and fibrinogen. Lab. Invest 14: 335 1965;
• 11 Caen J. P. Is fibrinogen the plasma co-factor in ADP-induced human platelet aggregation?. Nature (Paris) 205: 1120 1965;
• 12 Deykin D, Pritzker G. R, Scolnick E. M. Plasma co-factors in adenosine diphosphate- induced aggregation of human platelets. Nature (Lond) 208: 296 1965;
• 13 Evans G, Packham M. A, Nishizawa E. E, Mustard J. F, Murphy E. A. The effect of acetylsalicylic acid on platelet function. J. exp. Med 128: 877 1965;
• 14 Grossman M. I, Matsumoto K. K, Lichter R. J. Fecal blood loss produced by oral and intravenous administration of various salicylates. Gastroenterology 40: 383 1961;
• 15 Gugler E, Lüscher E. F. Platelet function in congenital afibrinogenemia. Thrombos. Diathes. haemorrh. (Stuttg) 14: 361 1965;
• 16 Hawkins D, Pinckard R. N, Farr R. S. Acétylation of human serum albumin by acetylsalicylic acid. Science 160: 780 1968;
• 17 Hawkins D, Pinckard R. N, Crawford I. P, Farr R. S. Structural changes in human serum albumin induced by ingestion of acetylsalicylic acid. J. clin. Invest 48: 536 1969;
• 18 Kaneshiro M. M, Mielke Jr C. H, Kasper G. K, Rapaport S. I. Bleeding time after aspirin in disorders of intrinsic clotting. N. Engl. J. Med 281: 1039 1969;
• 19 Keller W. J. A rapid method for the determination of salicylates in serum or plasma. Amer. J. clin. Path 17: 415 1947;
• 20 McLean J. R, Maxwell R. E, Hertler D. Fibrinogen and adenosine diphosphate-induced aggregation of platelets. Nature (Lond) 202: 605 1964;
• 21 Quick A. J. Salicylates and bleeding. The aspirin tolerance test. Amer. J. med. Sci 252: 265 1966;
• 22 Ödegaard A. E, Skàlhegg B. A, Hellem A. J. ADP-induced platelet adhesiveness as a diagnostic test in von Willebrand’s disease. Thrombos. Diathes. haemorrh. (Stuttg) 11: 23 1964;
• 23 Rowland M, Riegelman S. Pharmacokinetics of acetylsalicylic acid and salicylic acid after intravenous administration in man. J. pharm. Sci 57: 1313 1968;
• 24 Weiss H. J, Aledort L. M, Kochwa S. The effect of salicylates on the hemostatic properties of platelets in man. J. clin. Invest 47: 2169 1968;
• 25 Zucker M. B, Peterson J. Inhibition of adenosine diphosphate-induced secondary aggregation and other platelet functions by acetylsalicylic acid ingestion. Proc. Soc. exp. Biol. (N. Y) 127: 547 1968;