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Thromb Haemost 1970; 23(02): 261-275
DOI: 10.1055/s-0038-1654139
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

Assessment of Damage to Human Platelets after Aggregation and Other Injuries by Microscopic Observation and Estimation of Serotonin Uptake

G Zbinden   1
,
J. N Mehrishi
*   Departments of Medicine and Radiotherapeutics*, University of Cambridge, Cambridge
,
S Tomlin
› Institutsangaben
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Publikationsdatum:
24. Juli 2018 (online)

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Summary

The severity of platelet damage induced by hyper- and hypotonic NaCl solutions and freezing and thawing was assessed by microscopic evaluation and measuring inhibition of 5-HT uptake. The same techniques were used to quantitate the effects of aggregating agents. The positively charged macromolecules PS, Poly-L und Poly-O reduced the net negative surface charge as determined by microelectrophoresis, caused platelet aggregation and inhibited 5-HT uptake. The damaging effects of Poly-L and Poly-O were more severe and more closely related to concentration than that of PS. The negatively charged macromolecules Poly-IC and NaPS increased the anodic electrophoretic mobility. Poly-IC and heparin caused a low degree of platelet clumping and no inhibition of 5-HT uptake. NaPS produced severe platelet damage with extensive clumping and complete inhibition of 5-HT uptake. Na laurate had the same effect, but did not alter electrophoretic mobility. ADP caused concentration-dependent platelet aggregation and inhibition of 5-HT uptake. The effects of ADP and NaPS were compared in agitated and non-agitated platelet samples containing identical concentrations of the 2 compounds. Agitation was found to increase the degree of platelet clumping and to reduce 5-HT uptake.

1 Present address : Institute of Pathological Anatomy, University of Zurich, Zurich, Switzerland.


 

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