Effects on Fibrin Clot Lysis of a Trypsin Inhibitor in Peanuts (Arachis hypogaea)^[*]

Summary

Using thrombelastography the effects of a partially purified trypsin inhibitor from peanuts (Arachis hypogaea) on fibrinolysis and blood coagulation was investigated. It delays fibrinolysis induced by plasmin and by activators of plasminogen (urokinase and SK-activator). Assayed on plasmin the peanut inhibitor (PNTI) is slightly less active than soy bean trypsin inhibitor (SBTI) when equal antitryptic strengths are compared. PNTI is a more potent inhibitor of activator induced fibrinolysis than SBTI. The antifibrinolytic effect decreased in presence of human plasma. The thrombin generation in recai cified plasma was delayed by PNTI using about 30-fold the antifibrinolytic active concentration. There was only slight effect on tissue thromboplastin and no effect on thrombin in the concentrations used.

^* Supported by grant HE-05020 to Dr. Tage Astrup, Director of Research, from the U. S. Public Health Service, National Institutes of Health, National Heart Institute.

^** Present address : Surgical Department H., Copenhagen County Hospital, Gentofte, Denmark.

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