Fluid Mechanical Stress and the Platelet^[*]

Summary

Platelet thrombi in suspensions stirred in an aggregometer or subjected to shear flow in a Couette viscometer or circular tube can only form as a result of collisions between the cells. In whole blood, the presence of the red cells results in pronounced lateral displacements of the paths of the platelets through the vessel, which in turn increases the frequency of collisions between platelets and also makes possible platelet-wall interactions. In addition, blood cells in a suspension undergoing shear flow are subjected to fluid stresses which can result in their deformation. Such stresses may locally attain high values in the arterial circulation at bifurcations, sharp bends and stenoses where the flow is disturbed. Since platelet thrombi have been found downstream of sites of such geometry, the effects of high and variable shear stress on the platelet release reaction and aggregation were investigated. Washed platelets containing C¹⁴-serotonin in Tyrodes-albumin solution were subjected to oscillatory flow at 37° C and provided the time average wall velocity gradient was above 2,000 sec^-1, the thrombin-induced release of serotonin was appreciably greater than in the corresponding controls incubated at rest. No measurable serotonin was released as a result of flow alone. This synergistic effect of oscillatory shear flow was also noted in the presence of inhibitors of the release reaction.

^* Presented at the National Symposium on Thrombosis, Dallas, November 22, 1974.

• References

• 1 Batchelor G. K, and Green J. F. 1972; The hydrodynamic interaction of two small freely-moving spheres in a linear flow field. Journal of Fluid Mechanics 36: 375.
• 2 Blackshear P. L, Forstrom R. J, Lorberbaum M, Gott V. L, and Sovilj R. 1971 A role of flow separation and recirculation in thrombus formation on prosthetic surfaces. 9th Aerospace Sciences Meeting. American Institute of Aeronautics and Astronautics, New York. Paper 71-103.
• 3 Fox J. A, and Hugh A. E. 1966; Localization of atheroma. A theory based on boundary layer separation. British Heart Journal 28: 388.
• 4 Frojmovic M. M. 1973; Quantitative parameterization of the light transmission properties of citrated, platelet-rich plasma as a function of platelet and ADP concentrations and. temperature. Journal of Laboratory and Clinical Medicine 82: 137.
• 5 Frojmovic M. M, Okagawa A, and Mason S. G. 1975; Rheo-optical transients in erythrocyte suspensions. Biochemical and Biophysical Research Communications 62: 17.
• 6 Geissinger H. D, Mustard J. F, and Rowsell H. C. 1962; The occurrence of micro-thrombi on the aortic endothelium of swine. Canadian Medical Association Journal 87: 405.
• 7 Goldsmith H. L. 1967; The microscopic flow properties of red cells. Federation Proceedings 26: 1813.
• 8 Goldsmith H. L. 1971; Red cell motions and wall interactions in tube flow. Federation Proceedings 30: 1578.
• 9 Goldsmith H. L. 1974; Blood flow and thrombosis. Thrombosis et Diathesis Haemor-rhagica 32: 35.
• 10 Goldsmith H. L, and Mason S. G. 1967. The microrheology of dispersions. In: Eirich F. R. (ed.) Eheology: Theory and Applications. Vol. IV. Academic; New York: 85.
• 11 Goldsmith H. L, and Mason S. G. 1971. Some model experiments in hemodynamics. IV. In: Hartert H. H, and Copley A. L. (eds.) Theoretical and Clinical Hemo-rheology. Springer; New York: 47.
• 12 Goldsmith H. L, and Mason S. G. 1975 Some model experiments in hemodynamics. V. Microrheological techniques. Biorheology. 12. In Press.
• 13 Goldsmith H. L, and Marlow J. 1972; Flow behaviour of erythrocytes. I. Rotation and deformation in dilute suspensions. Proceedings of the Royal Society London B182: 351.
• 14 Goldsmith H. L, and Marlow J. 1975 Flow behaviour of erythrocytes. II. Velocity distributions, paths and cell deformation in concentrated suspensions. To be submitted.
• 15 Goldsmith H. L, and Skalak R. 1975; Hemodynamics. Annual Reviews of Fluid Mechanics 7: 213.
• 16 Karnis A, Goldsmith H. L, and Mason S. G. 1966; The kinetics of flowing dispersions. I. Concentrated suspensions of rigid particles. Journal of Colloid and Interface Science 22: 531.
• 17 Klose J. J, Rieger H, Heinich L, and Schmid-Schönbein H. 1975 A new method for the study of platelet rheology in artificial flow. Thrombosis Research. (In Press).
• 18 Kwaan H. C, Harding F, and Astrup T. 1967. Platelet behavior in small blood vessels in vivo . In: Brinkhous et al. (eds.) Platelets: Their role in He?nostasis and Thrombosis. Thrombosis et Diathesis Haemorrhagica, Suppl. 26. Schattauer Verlag; Stuttgart: 208.
• 19 Ling S. C, Atabeck H. B, Fry D. L, Patel D. J, and Janicki J. S. 1968; Application of heated-film velocity and shear probes to hemodynamic studies. Circulation Research 23: 789-801
• 20 Maccallum R. N, O’Bannon W, Hellums J. D, Alfrey C. P, and Lynch E. C. 1973. Viscometric instruments for studies on red blood cell damage. In: Gabelnick and Litt. (eds.) Rheology of Biological systems. Thomas; Springfield, Illinois: 70.
• 21 Mitchell J. R. A, and Schwartz C. J. 1963; The relationship between myocardial lesions and coronary artery disease. II. A selected group of patients with massive cardiac necrosis or scarring. British Heart Journal 25: 1-24
• 22 Mustard J. F, Perry D. W, Ardlie 1St G, and Packham M. A. 1972; Preparation of suspensions of washed platelets from humans. British Journal of Haematology 22: 193.
• 23 O’Brien J. R. 1962; Platelet aggregation. II. Some results from a new method of study. Journal of Clinical Pathology (London) 15: 452.
• 24 Palmer A. A. 1967. Platelet and leucocyte skimming. Bibliotheca Anatomica, 9, 300. Karger; New York:
• 25 Schmid-Schönbeln H, and Wells R. 1969; Fluid drop-like transition of erythrocytes under shear. Science 165: 288.
• 26 Schmid-Schönbein H, Gosen J. v, Heinich L, Klose H. J, and Volger E. 1973; A counter-rotating “rheoscope chamber” for the study of the microrheology of blood cell aggregation by microscopic observation and microphotometry. Microvascular Research 6: 366.
• 27 Schultz D. L, Tunstall-Pedoe D. S, Lee G. de J, and Bellehouse B. J. 1969. Velocity distribution and transition in the arterial system. In: Wostenholme and Knight. (eds.) Circulatory and Respiratory Mass Transport. Churchill; London: 172.
• 28 Vadas E. B, Goldsmith H. L, and Mason S. G. 1973; The microrheology of colloidal dispersions. I. The microtube technique. Journal of Colloid and Interface Science 43: 630.
• 29 Vasseur P. 1973 The lateral migration of spherical particles in a fluid bounded by parallel plane walls. Ph. D. Thesis. McGill University, Montreal.
• 30 Womersley J. R. 1955; Method for the calculation of velocity, rate of flow and viscous drag in arteries when the pressure gradient is known. Journal of Physiology 127: 553.
• 31 Yu S. K. 1973 The behavior of model particles and blood cells in a vortex, and the effects of shear on the platelet release reaction. Ph. D. Thesis. McGill University, Montreal.
• 32 Yu S. K, and Goldsmith H. L. 1973; Behavior of model particles and blood cells at spherical obstructions in tube flow. Microvascular Research 6: 5.
• 33 Yu S. K, and Goldsmith H. L. 1975. Some rheological aspects of platelet thrombosis. In: Hirsh. (ed.) Platelets, Drugs and Thrombosis. Karger; Basel: 78.
• 34 Yu S. K, Marlow J. C, and Goldsmith H. L. 1975 The effect of oscillatory flow on the release reaction and aggregation of human platelets. To be submitted.
• 35 Zucker M. B, Rifkin P. L, Friedberg N. M, and Coller B. S. 1972; Mechanisms of platelet function as revealed by retention of platelets in glass bead columns. Annals of the New York Academy of Sciences 201: 138.