Kinetic Aspects of the Interaction of Blood Clotting Enzymes

H. C Hemker; A. D Muller

doi:10.1055/s-0038-1651215

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1968; 19(03/04): 368-382
DOI: 10.1055/s-0038-1651215

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

V. The Reaction Mechanism of the Extrinsic Clotting System as Revealed by the Kinetics of One-Stage Estimations of Coagulation Enzymes

H. C Hemker

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Depts. of Haematology and Cardiology, Clinic for Internal Medicine, University Hospital, Leiden, The Netherlands

,

A. D Muller

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Depts. of Haematology and Cardiology, Clinic for Internal Medicine, University Hospital, Leiden, The Netherlands

› Author Affiliations

Abstract

Summary

Observations of the clotting time in one-stage tests in the extrinsic system show, after suitable correction, that there is a rectilinear relationship between the clotting time and the inverse of the concentration of the rate-limiting factor. This cannot be explained by the “cascade” or “waterfall” mechanism proposed as a scheme for the interaction of the blood-clotting factors concerned. This relationship can, however, be explained by a slightly different mechanism.

Full Text

References

References
1 Berry G.G. Antithromboplastin The degeneration of intrinsic thromboplastin in normal serum. J. clin. Path. 1958; 11: 39
2 Biggs R, Douglas A.S, Macfarlane R.G. The formation of thromboplastin in human blood. J. Physiol. (Lond.) 1953; 119: 89
3 Biggs R, Macfarlane R.G. Human blood coagulation and its disorders. Blackwell Scientific Publications; Oxford: 1962
4 Breckenridge R.T, Ratnojf O.D. The activation of human pro-accelerin (Factor V). Thrombos. Diathes. haemorrh. (Stuttg.) Suppl. 1965; 17: 218
5 Breckenridge R.T, Ratnojf O.D. The activation of pro-accelerin by bovine thrombokinase (with notes on the antithrombic activity of phenylmethylsulfonylfluoride). Blood 1966; 27: 527
6 Davie E.W, Ratnojf O.D. A waterfall sequence for intrinsic blood clotting. Science 1964; 145: 1310
7 Deutsch E, Fuchs H. Natürlich vorkommende Koagulationsinhibitoren. Acta haemat. (Basel) 1958; 20: 97
8 Deutsch E, Mammen F. The inactivation of plasma-thromboplastin. Thrombos. Diathes. haemorrh. (Stuttg.) 1958; 2: 324
9 Dixon M, Webb E. Enzymes. Longmans, Green & Co.; London: 1964
10 Egeberg O. On the natural blood coagulation inhibitor system. Investigations of inhibitory factors based on antithrombin deficient blood. Thrombos. Diathes. haemorrh. (Stuttg.) 1965; 14: 473
11 Egli H, Kesseire K, Klesper R. Über die Inaktivierung von Blutthrombokinase. Acta haemat. (Basel) 1957; 17: 338
12 Esnouf M.P, Jobin F. Studies on the formation of the prothrombin converting complex. Biochem. J. 1967; 102: 666
13 Hermansky F, Vitek J. The role of proconvertin and Stuart factor in the inactivation of tissue thromboplastin by serum. Experientia (Basel) 1960; 16: 455
14 Hemker H.C, Hemker P.W, Loeliger E.A. Kinetic aspects of the interaction of blood clotting enzymes. I. Derivation of basic formulas. Thrombos. Diathes. haemorrh. (Stuttg.) 1965; 13: 155
15 Hemker H.C, Esnouf P.W, Hemker P.W, Swart A.G.W, Macfarlane R.G. Formation of prothrombin converting activity. Nature (Lond.) 1967; 215: 248
16 Hemker H.C, Loeliger E.A, Veltkamp J.J. Kinetic aspects of the interaction of blood-clotting enzymes. III. Demonstration of an inhibitor of prothrombin conversion in vitamin K deficiency. Thrombos. Diathes. haemorrh. (Stuttg.) 1968; 19: 346
17 Hemker H.C, Siepel T.V, Altman R, Loeliger E.A. Kinetic aspects of the interaction of blood-clotting enzymes. II. The relation between clotting time and plasma concentration in prothrombin-time estimations. Thrombos. Diathes. haemorrh. (Stuttg.) 1967; 17: 349
18 Hemker H.C, van der Meer J, Loeliger E.A. Kinetic basis of prothrombin estimation. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl. 1965; 17: 247
19 Hemker H.G, Hemker P.W. Kinetics of the interaction of blood clotting factors in a purified system. Abstract F. E. B. S. meeting Oslo 13:49; 1967; 13: 50
20 Hemker H.C. in discussion to R G. Macfarlane. A clotting scheme for 1964. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl. 1965; 17: 63
21 Hensen A, Loeliger E.A. Antithrombin III. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 10 1963
22 Jürgens J. Factor VII-inhibitor. A new physiological serum accelerator inactivation principle. Acta haemat. (Basel) 1955; 14: 57
23 Macfarlane R.G. An enzyme cascade in blood coagulation. Nature (Lond.) 1964; 202: 498
24 Papahadjopoulos D, Hanahan D.J. Observations on the interactions of phospholipids and certain clotting factors in prothrombin activator formation. Biochim. biophys. Acta (Amst.) 1864; 90: 436
25 Prentice C.R.M, Breckenridge R.T, Ratnoff O.D. Studies on the conversion of prothrombin to thrombin (with notes on the cation requirement for this reaction). J. Lab. clin. Med. 1967; 69: 229
26 Seegers W.H. Basic enzymology of blood coagulation. Thrombos. Diathes. haemorrh. (Stuttg.) 1965; 14: 213
27 Seegers W.H. Prothrombin. Harvard University Press; Cambridge, Massachusetts: 1962
28 Shinowara G.Y. Human thrombin and fibrinogen. The kinetics of their interaction and the preparation of the enzyme. Biochim. biophys. Acta (Amst.) 1966; 113: 359
29 Soulier J.P. Les inhibiteurs naturaux de la coagulation. Sang 1959; 30: 262
30 Soulier J.P. Natural inhibitors. Progress in coagulation. F. K. Schattauer; Stuttgart: 1962: 38 p.
31 Spaet T.H, Garner E.S. Inactivation of thromboplastin in human blood. J. clin. Invest. 1955; 54: 1807
32 Straub W, Duckert F. The formation of the extrinsic prothrombin activator. Thrombos. Diathes. haemorrh. (Stuttg.) 1961; 5: 402