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Thromb Haemost 1972; 27(02): 349-360
DOI: 10.1055/s-0038-1649373
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

Reptilase-Induced Shortening of Coagulation Times in Normal and Hemophilic Individuals[*]

A. J Harder
1   Department of Medicine, Kantonsspital, University of Zürich, Switzerland
,
H Stadelmann
1   Department of Medicine, Kantonsspital, University of Zürich, Switzerland
,
P. W Straub
1   Department of Medicine, Kantonsspital, University of Zürich, Switzerland
› Author Affiliations
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Publication History

Publication Date:
29 June 2018 (online)

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Summary

In 5 normal volunteers infusion of small doses of Reptilase-def., a thrombin-like snake venom enzyme of Bothrops atrox, led to a transient shortening of the clotting time, which was significant when compared with a control group infused with glucose. In hemophilic individuals, clotting and recalcification times were also considerably shortened, the maximal effect in both groups occurring simultaneously with cryoprecipitability and positive paracoagulation phenomena. Whether this accelerating effect on blood coagulation is mediated by circulating soluble fibrin complexes, is not yet established. However, the clot-promoting action of Reptilase must predominantly be localized at the level of fibrinogen/fibrin conversion rather than in earlier phases of coagulation.

* Presented at the V. Annual Meeting, European Society for Clinical Investigation, Sch eveningen, The Netherland, April 22-24, 1971.


 

  • References

  • 1 Astrujp T, Müllertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem 40: 346 1952;
    CrossrefPubMedSearch in Google Scholar
  • 2 Bell W. R, Pitney W. R, Goodwin J. F. Therapeutic defibrination in the treatment of thrombotic disease. Lancet I: 490 1968;
    PubMedSearch in Google Scholar
  • 3 Blombäck B. Studies on the action of thrombic enzymes on bovine fibrinogen as measured by N-terminal analysis. Arkiv for Kemi 12: 321 1958;
    PubMedSearch in Google Scholar
  • 4 Blombäck B, Blombäck M, Nilsson I. M. Coagulation studies on “Reptilase”, an extract of the venom from Bothrops jararaca. Thrombos. Diathes. haemorrh. (Stuttg) 01: 76 1957;
    PubMedSearch in Google Scholar
  • 5 Blombäck M, Egberg N, Gruder E, Johansson S. A, Johansson H, Nilsson S. E. G , Blombäck A. Treatment of thrombotic disorders with Reptilase. Annual Meeting of Int. Soc. Thrombosis and Hemostasis. Montreux, July 1970. Thrombos. Diathes. haemorrh. (Stuttg) Suppl. 45: 51 1971;
    PubMedSearch in Google Scholar
  • 6 Bruhn H. D, Müller G, Duckert F. Quantitative determination of plasminogen. Thrombos. Diathes. haemorrh. (Stuttg) 23: 191 1970;
    PubMedSearch in Google Scholar
  • 7 Glauss A. Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Actahaemat. (Basel) 27: 237 1957;
    PubMedSearch in Google Scholar
  • 8 Duckert F. Le diagnostic des coagulopathies. 3. Congrès internat, de Biologie Clinique. Bruxelles. 1957: 635-654 Presses Académiques Européennes; Bruxelles: 1958
    Search in Google Scholar
  • 9 Durante L. J, Moutsos A, Ambrose R. B, Duncan W. J, Fleming W, Zinsser H. H, Phillips L. L. Prostatectomy bleeding. Ann. Surg 156: 781 1962;
    CrossrefPubMedSearch in Google Scholar
  • 10 Funk G, Gmür J, Herold R, Straub P. W. Reptilase, a new reagent in blood coagulation. Brit. J. Haemat 21: 43 1971;
    CrossrefPubMedSearch in Google Scholar
  • 11 Geiger M, Duckert F, Koller F. Quantitative Bestimmung von Faktor VIII and IX bei Blutersippen. Kongr. europ. Ges. Haemat., S. 413 Springer; Heidelberg: 1956
    Search in Google Scholar
  • 12 Godai C, Abildqaard U. Gelation of soluble fibrin in plasma by ethanol. Scand. J. Haemat 03: 342 1966;
    PubMedSearch in Google Scholar
  • 13 Harder A. J, Funk G, Straub P. W. In vivo and in vitro production by Reptilase of cryofibrinogen and paracoagulation: a model for subclinical intravascular coagulation?. XIII. Internat. Congr. Haemat., Munich. August 2-8. 1970 Abstract Volume 238.
    PubMedSearch in Google Scholar
  • 14 Hohen H. W. Experimentelle Studien zur Frage der Beeinflussung der Blutgerinnung durch Reptilase. Z. ges. exp. Med 128: 427 1957;
    CrossrefPubMedSearch in Google Scholar
  • 15 Jung E. G. A rapid quantitative assay of factor VIII (AHG) without the use of hemophilia A plasma. Thrombos. Diathes. haemorrh. (Stuttg) 04: 323 1960;
    PubMedSearch in Google Scholar
  • 16 Kalbfleisch J. M, Bird R. M. Cryofibrinogenemia. New Engl. J. Med 263: 881 1960;
    CrossrefPubMedSearch in Google Scholar
  • 17 Lipinski B, Latallo S, Kowalski E. Soluble unclottable complexes formed in the presence of FDP during the fibrinogen-fibrin conversion and their potential significance in pathology. Thrombos. Diathes. haemorrh. (Stuttg) 17: 65 1967;
    PubMedSearch in Google Scholar
  • 18 Merskey G, Kleiner G. J, Johnson A. J. Quantitative estimation of split products of fibrinogen in human serum. Relation to diagnosis and treatment. Blood 28: 1 1966;
    PubMedSearch in Google Scholar
  • 19 Penick G. D, Roberts H. R, Dejanov I. I. Covert intravascular clotting. Fed. Proc 24: 835 1965;
    PubMedSearch in Google Scholar
  • 20 Pitney W. R, Holt P. J. L , Bray G, Bolton G. Acquired resistance to treatment with Arvin. Lancet 01: 79 1969;
    PubMedSearch in Google Scholar
  • 21 Schulz F. Eine einfache Bewertungsmethode von Leberparenchymschäden (volumetrische Fibrinbestimmung). Actahepat. (Hamburg) 09: 306 1955;
    PubMedSearch in Google Scholar
  • 22 Shainoff J. R, Page I. H. Cofibrins and fibrin intermediates as indicators of thrombin activity in vivo. Circulât. Research 08: 1013 1960;
    PubMedSearch in Google Scholar
  • 23 Shainoff J. R, Page I. H. Significance of cryofibrin in fibrinogen-fibrin conversion. J. exp. Med 21 (05) 687 1962;
    PubMedSearch in Google Scholar
  • 24 Stacker A, Böhnel J. Experimentelle und klinische Untersuchungen zur hämostatischen Wirkung von Schlangengiften. Wien. klin. Wschr 19: 333 1959;
    PubMedSearch in Google Scholar
  • 25 Stocker K, Straub P. W. Rapid detection of fibrinopeptides by bidimensional paper electrophoresis. Thrombos. Diathes. haemorrh. (Stuttg) 24: 248 1970;
    PubMedSearch in Google Scholar
  • 26 Straub P. W, von Felten A, Frick P. G. Recurrent intravascular coagulation with renal cortical necrosis and recovery. Ann. intern. Med 64: 643 1966;
    CrossrefPubMedSearch in Google Scholar
  • 27 Sugano T, Maruyama R. Experiences of the use of Reptilase in the field of otorhinolaryngology. Otorhinolaryngology (Tokyo) 36: 365 1964;
    PubMedSearch in Google Scholar
  • 28 v. Kaulla K.N, Schultz R. L. Methods for the evaluation of human fibrinolysis. Amer. J. clin. Path 29: 104 1958;
    PubMedSearch in Google Scholar