Download PDF
Thromb Haemost 1972; 28(02): 228-236
DOI: 10.1055/s-0038-1649059
Original Article
Schattauer GmbH

Effect of Lidoflazine (R 7904) on Human Platelet Function in Vitro

F De Clerck
1   Cardiovascular Research Department Janssen Pharmaceutica Research Laboratories, Beerse, Belgium
› Author Affiliations
Further Information

Publication History

Publication Date:
24 July 2018 (online)

    Permissions and Reprints

Summary

The effect of lidoflazine and of cinnarizine on human platelet function in vitro was compared to that of dipyridamole.

Pre-incubation for 30 min at 37° C of platelet rich plasma with lidoflazine or with dipyridamole 5 ×10–4 M resulted in an appreciable inhibition of collagen aggregation in particular and to a lesser extent of ADP aggregation; cinnarizine was marginally active only.

Clot retraction was inhibited by lidoflazine and by dipyridamole. Experiments on biphasic ADP aggregation and C14-serotonin release during aggregation show that lidoflazine reduces the platelet release reaction.

The possible mode of action of the compound is discussed.

Plasma coagulation and PF – 3 availability were not affected.

 

  • References

  • 1 Aster R. M, and Jandl J. M. 1964; Platelet Sequestration in Man. I. Methods. Journal of Clinical Investigation 43: 843.
    CrossrefPubMedGoogle Scholar
  • 2 Baumgartner H. R, and Born G. V. R. 1968; Effects of 5-hydroxytryptamine on platelet aggregation. Nature 218: 137.
    CrossrefPubMedGoogle Scholar
  • 3 Born G. V. R, and Cross M. J. 1963; The aggregation of blood platelets. Journal of Physiology 168: 178.
    CrossrefPubMedGoogle Scholar
  • 4 Caen J, Larrieu M. J, and Samama M. L’hémostase. Méthodes d’exploration et diagnostique pratique. L’expansion Scientifique; Paris.:
    Google Scholar
  • 5 Dacie J. V, and Lewis S. M. 1968. Practical Haematology. J. & A. Churchill Ltd.; London:
    Google Scholar
  • 6 Dougherty W. J, and Bicher H. I. 1971; Effects of antiadhesive agents on the ultrastructure of rabbit blood platelets in vitro. Thrombosis et Diathesis Haemorrhagica 26: 378.
    PubMedGoogle Scholar
  • 7 Eliasson R, and Bygdeman S. 1969; Effect of dipyridamole and two pyrimido-pyrimidine derivatives on the kinetics of human platelet aggregation and on platelet adhesiveness. Scandinavian Journal of Clinical and Laboratory Investigations 24: 145.
    CrossrefPubMedGoogle Scholar
  • 8 Hampton J. R, Harrison M. J, Honour A. J, and Mitchell J. R. 1967; Platelet behaviour and drugs used in cardiovascular disease. Cardiovascular Research 1: 101.
    CrossrefPubMedGoogle Scholar
  • 9 Hassanein A. A, Turpie A. G, McNicol G. P, and Douglas A. S. 1970; Effect of RA 233 on platelet function in vitro. British Medical Journal 3: 83.
    PubMedGoogle Scholar
  • 10 Macmillan D. C, and Oliver M. F. 1965; The initial changes in platelet morphology following the addition of adenosine diphosphate. Journal of Arterosclerosis Research 5: 440.
    PubMedGoogle Scholar
  • 11 Schaper W. K. A, Xhonneux R, Jageneau A. H. M, and Janssen P. A. J. 1966; The cardiovascular pharmacology of lidoflazine, a long-acting coronary vasodilator. Journal of Pharmacology and Experimental Therapeutics 152: 265.
    PubMedGoogle Scholar
  • 12 Spaet T. H, and Cintron J. 1965; Studies on platelet factor-3 availability. British Journal of Haematology 2: 269.
    PubMedGoogle Scholar
  • 13 Van Belle H. 1970; The disappearance of adenosine in blood. Effect of lidoflazine and other drugs. European Journal of Pharmacology 11: 241.
    CrossrefPubMedGoogle Scholar