Patterns of Incorporation of ⁷⁵Se-Methionine into Fibrinogen and Other Plasma Proteins in Rabbits Stimulated by Different Test Conditions

Summary

Rabbits were injected with ⁷⁵Se-Methionine (⁷⁵SeM) 4-8 hr after being subjected to a variety of experimental conditions: injection of ACTH, growth hormone, glucagon, adrenalin, endotoxin, turpentine, hydrocortisone and laparotomy. All of these experimental conditions except injection of glucagon were associated with increased incorporation of ⁷⁵SeM into fibrinogen. Three patterns of incorporation of ⁷⁵SeM into plasma proteins were recognized: 1. the pituitary pattern, which was observed in animals injected with ACTH, growth hormone or endotoxin, and which was characterized by increased incorporation of ⁷⁵SeM only into fibrinogen and by a delayed incorporation of ⁷⁵SeM into α₂ and β₁ globulins; 2. the tissue injury pattern, which was characterized by a markedly increased incorporation of ⁷⁵SeM into fibrinogen and no alteration in incorporation of ⁷⁵SeM into α₂ or β₁ globulins; and 3. the pharmacologic corticosteroid pattern, which was characterized by a moderately increased incorporation of ⁷⁵SeM into fibrinogen and a strikingly increased incorporation of ⁷⁵SeM into α₂ and β₁ globulins.

^* Present Address : Department of Hematology, Chaim Sheba Medical Center, Tel-Hashomer, Israel.

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