Isolation of the ⁶⁷Gallium Accumulating Fraction in Normal Rat Liver

Summary

Subcellular ⁶⁷Gallium distribution was investigated in normal rat liver after intravenous injection. By differential centrifugation and density gradient centrifugation ⁶⁷Gallium accumulating bodies were isolated and identified as lysosomes by enzyme determination and electron microscopy. ⁶⁷Gallium enrichment in this fraction was 23-fold. Using the isolated ⁶⁷Gallium accumulating lysosomes the binding state of the isotope inside the lysosomes was studied. ⁶⁷Gallium was found to be associated with the soluble fraction of lysosomes.

Die subzelluläre Verteilung von ⁶⁷Gallium wurde in der normalen Rattenleber nach intravenöser Tracer-Injektion untersucht. Mit Hilfe der Differentialzentrifugation sowie der Dichtegradientenzentrifugation konnte eine ⁶⁷Ga-akkumulierende Fraktion isoliert werden, die eine 23fache ⁶⁷Ga-Anreicherung gegenüber dem Gesamthomogenat aufwies. Die ⁶⁷Ga-anreichernden Partikel wurden enzymatisch sowie elektronenoptisch als Lysosomen identifiziert.

An den isolierten ⁶⁷Ga-reichen Lysosomen wurde die intralysosomale Verteilung des Isotops näher untersucht. Es zeigte sich dabei, daß das ⁶⁷Gallium nicht an die lysosomale Membran oder an Strukturproteine gebunden vorliegt, sondern sich in der löslichen Fraktion der Lysosomen befindet.

^* N.V. Philips Duphar Cyclotron and Isotope Laboratories, Petten, Netherlands. 1 ml of the original solution contained 1.25-5 mg sodium citrate, 9.0 mg benzyl alkohol, 1-2 mCi ⁶⁷Ga (first day) carrier-free, phenol red as indicator.

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