Venoneuronale De- und Regeneration bei Varikogenese und Neovaskularisation

Zusammenfassung

Ziel: Der Ausschluss von Neovaskulaten via S100-Protein ist ungenau. Geklärt werden sollte, ob NGF (nerve growth factor) valider ist, zumal eine angiogenetische Potenz besteht. Methode: Nervenfaserfärbung gesunder (n = 16), variköser (n = 110) und von Rezidivvenen der Krosse (n = 24) mit S100- und NGF-Antikörpern (AK). Semiquantitative Evaluation der jeweiligen Nervenfaserdichte, Quotientenbildung zur Darstellung der Unterschiede. Zusätzlich Evaluation des prozentualen Anteils gefärbter Präparate. Ergebnisse: In allen Gruppen Nachweisbarkeit adventitieller Nervenfasern mit beiden AK. In Kontroll- und Varikosisgruppe war die NGF-Faserdichte kleiner als bei S100. Bei Rezidiven erreichte die NGF-Faserdichte Werte wie bei S100. Gegensätzlich zur NGF-Faserdichte sind S100-Werte bei Rezidiven kleiner, als bei S100-Kontrollen. Geringste Faserdichte für beide Proteine in der Varikosisgruppe. S100-Rezidive hatten den geringsten Anteil anfärbbarer Fasern. Schlussfolgerung: NGF könnte die venoneuronale Regeneration triggern und als Marker spezifischer als S100 sein. Die Varikogenese könnte durch eine reduzierte Venenwandinnervation (venoneuronale Degeneration) induziert werden.

Summary

The exclusion of neovascularisation via S100 protein is inexact. The aim was to clarify whether NGF (nerve growth factor) is more valid, especially as it has angiogenetic potency. Method: Staining of nerve fibres from healthy (n = 16), varicose (n = 110) and recurrent varicose veins of the saphenofemoral junction (n = 24) with S100 and NGF antibodies (AB). Semiquantitative evaluation of the respective nerve fibre density and ratio calculation to illustrate the differences. In addition, evaluation of the percentage of stained specimens. Results: In all groups, evidence of adventitial nerve fibres with both AB. In the control and varicose veins group, the NGF fibre density was lower than the S100 fibre density. In the recurrent varicose veins group, the NGF fibre density reached similar values to those of S100. Unlike the NGF fibre density, the values obtained for S100 in the recurrent varicose veins group are lower than those of S100 controls. Lowest fibre density for both proteins in the varicose veins group. Recurrent varicose veins stained with S100 antibodies had the lowest percentage of stainable fibres. Conclusion: NGF could trigger venoneuronal regeneration and be a more specific marker than S100. Varicogenesis could be induced by reduced innervation of the vessel wall (venoneuronal degeneration).

Résumé

But : l’interprétation de la néovascularisation par la protéine S-100 est controversée. La question est posée de savoir si le potentiel angiogénétique peut être déterminé par le FCN. Méthode : coloration des fibres nerveuses par des anticorps anti S-100 et anti FCN sur des veines saines (n = 16), variqueuses (n = 110) et sur des cas de récidive de la crosse (n = 24). Evaluation semi-quantitative de l’épaisseur des fibres nerveuses pour évaluation des différences du potentiel de coloration. En outre, évaluation du pourcentage de coloration des préparations. Résultat : appréciation dans les deux groupes de l’imprégnation adventitielle par les deux méthodes. L’épaisseur des fibres, évaluée par le FCN était plus petite que par la protéine S-100 dans le groupe de contrôle et dans le groupe variqueux. En cas de récidive variqueuse, les deux méthodes sont analogues. A l’opposé, l’épaisseur des fibres est plus petite dans les récidives que dans les contrôles, par la protéine S-100. Les fibres les plus minces contrôlées par les deux protéines ont été vues dans le groupe des veines variqueuses. Les varices récidivantes ont montré la plus petite portion de fibres colorées dans la méthode avec la protéine S-100. Conclusion : le FCN pourrait stimuler la régénération veino-neuronale et pourrait être un marqueur plus spécifique que la protéine S-100. La genèse variqueuse pourrait être induite par une innervation diminuée de la paroi veineuse (dégénérescence veino-neuronale).

Schlüsselwörter

NGF - Nerve-growth-Faktor - S100-Protein - Rezidivvarikosis - Neovaskularisation - Nervenfaserregeneration

Keywords

NGF - nerve growth factor - S100 protein - recurrent varicose veins - neovascularisation - regeneration of nerve fibers

Mots clés

Facteur de croissance nerveuse - FCN - protéine S-100 - récidive variqueuse - néovascularisation - régénération de fibres nerveuses

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