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Vet Comp Orthop Traumatol 2007; 20(01): 34-37
DOI: 10.1055/s-0037-1616585
Original Research
Schattauer GmbH

The effects of radiofrequency energy probe speed and application force on chondrocyte viability

Y. Lu
1   Comparative Orthopaedic Research Laboratory, Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA
,
M. L. Meyer
1   Comparative Orthopaedic Research Laboratory, Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA
,
J. J Bogdanske
1   Comparative Orthopaedic Research Laboratory, Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA
,
M. D. Markel
1   Comparative Orthopaedic Research Laboratory, Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA
› Author Affiliations
Further Information

Publication History

Received 11 April 2006

Accepted 04 July 2006

Publication Date:
21 December 2017 (online)

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Summary

Objective: To determine the thermal effects of monopolar radiofrequency energy (mRFE) on bovine articular cartilage when it was moved at different speeds and using varying application forces.

Methods: Thirty-six fresh osteochondral sections divided into two groups (18 sections/group) were used in this study. The first group was tested at three speed rates of mRFE probe (1 mm/sec, 5 mm/sec and 10 mm/sec) at a constant force (50 g) applied to the probe tip. In the second group, three application forces of the probe tip were tested (25 g, 50 g and 75 g) at a constant speed (5 mm/sec) (n=6/test). All tests were performed using a custom-built jig to control the mRFE (Vulcan EAS™) probe during a 20-mm pass on each section. After treatment, viability of osteochondral sections was determined by confocal laser microscopy (CLM) combined with vital cell staining.

Results: There were not any significant differences in cartilage thickness of tested osteochondral sections among the different speeds or forces. During the mRFE probe treatments at different speeds, CLM demonstrated that probe application at the speed of 1 mm/ sec caused significantly greater chondrocyte death than at the speeds of 5 and 10 mm/sec, whereas there were no significant differences in chondrocyte death among the variable application forces (p>0.05).

Discussion: This in vitro study demonstrated that RFE thermal penetration correlated most closely with probe application speed than application force for this mRFE probe.

Clinical relevance: Improper use of mRFE may cause thermal injury on articular cartilage.

Keywords

Chondrocyte - articular cartilage - radiofrequency energy - confocal laser microscopy - thermal chondroplasty
 

  • References

  • 1 Edwards RB, Lu Y, Kalscheur VL. et al. Thermal chondroplasty of chondromalacic human cartilage: an ex vivo comparison of bipolar and monopolar radiofrequency devices. Am J Sports Med 2002; 30: 90-97.
    CrossrefPubMedGoogle Scholar
  • 2 Hogan CJ, Diduch DR. Progressive articular cartilage loss following radiofrequency treatment of a partial-thickness lesion. Arthroscopy. 2001 17. E24.
    PubMedGoogle Scholar
  • 3 Kaplan L, Uribe JW, Sasken H. et al. The acute effects of radiofrequency energy in articular cartilage: an in vitro study. Arthroscopy 2000; 16: 2-5.
    CrossrefPubMedGoogle Scholar
  • 4 Lu Y, Hayashi K, Hecht P. et al. The effect of monopolar radiofrequency energy on partial-thickness defects of articular cartilage. Arthroscopy 2000; 16: 527-536.
    CrossrefPubMedGoogle Scholar
  • 5 Shellock FG, Shields CL. Radiofrequency energy-induced heating of bovine articular cartilage using a bipolar radiofrequency electrode. Am J Sports Med 2000; 28: 720-724.
    CrossrefPubMedGoogle Scholar
  • 6 Turner AS, Tippett JW, Powers BE. et al. Radiofrequency (Electrosurgical) ablation of articular cartilage. a study in sheep. Arthroscopy 1998; 14: 585-591.
    PubMedGoogle Scholar
  • 7 Li S, Chien S, Branemark P. Heat Shock-induced necrosis and apoptosis in osteoblasts. J Orthop Res 1999; 17: 891-899.
    CrossrefPubMedGoogle Scholar
  • 8 Zuger BJ, Ott B, Mainil-Varlet P. et al. Laser solder welding of articular cartilage: tensile strength and chondrocyte viability. Lasers Surg Med 2001; 28: 427-434.
    CrossrefPubMedGoogle Scholar
  • 9 Lu Y, Au E, Meyer ML. et al. Prototype radiofrequency probe reduces chondrocyte death compare to current monopolar and bipolar devices for treatment of chondromalacia. Proceed. 49th Annual Meeting of Orthopedic Research Society. 2003 49. 688.
    PubMedGoogle Scholar
  • 10 Amiel D, Ball ST, Tasto JP. Chondrocyte viability and metabolic activity after treatment of bovine articular cartilage with bipolar radiofrequency: an in vitro study. Arthroscopy 2004; 20: 503-510.
    CrossrefPubMedGoogle Scholar
  • 11 Meyer ML, Lu Y, Markel MD. Effects of radiofrequency energy on human chondromalacic cartilage: an assessment of insulation material properties. IEEE Trans Biomed Eng 2005; 52: 702-710.
    CrossrefPubMedGoogle Scholar
  • 12 Lu Y, Edwards III RB, Nho S. et al. Thermal chondroplasty with bipolar and monopolar radio frequency energy: Effect of treatment time on chondrocyte death and surface contouring. Arthroscopy 2002; 18: 779-788.
    CrossrefPubMedGoogle Scholar
  • 13 Lu Y, Edwards RB, Nho S. et al. Lavage solution temperature influences depth of chondrocyte death and surface contouring during thermal chondroplasty with temperature controlled monopolar radiofrequency energy. Am J Sports Med 2002; 30: 667-673.
    CrossrefPubMedGoogle Scholar
  • 14 Lu Y, Edwards RB, Cole BJ. et al. Thermal chondroplasty with radiofrequency energy: An in vitro comparison of bipolar and monopolar radio frequency devices. Am J Sports Med 2001; 29: 42-49.
    CrossrefPubMedGoogle Scholar
  • 15 Ohlendorf C, Tomford WW, Mankin HJ. Chondrocyte survival in cryopreserved osteochondral articular cartilage. J Orthop Res 1996; 14: 413-416.
    CrossrefPubMedGoogle Scholar
  • 16 Ryan A, Bertone AL, Kaeding CC. et al. The effects of radiofrequency energy treatment on chondrocytes and matrix of fibrillated articular cartilage. Am J Sports Med 2003; 31: 386-391.
    CrossrefPubMedGoogle Scholar
  • 17 Organ Organ. Electrophysiologic principles of radio frequency lesion making. Appl Neurophysiol 1976; 39: 69-76.
    PubMedGoogle Scholar
  • 18 Hunziker Hunziker, Quran TM. Surgical removal of articular cartilage leads to loss of chondrocytes from cartilage bordering the wound edge. J Bone Joint Surg Am 2003; 85 Suppl 2 85-92.
    PubMedGoogle Scholar
  • 19 Tew SR, Kwan AP, Hann A. et al. The reactions of articular cartilage to experimental wounding: role of apoptosis. Arthritis Rheum 2000; 43: 215-225.
    CrossrefPubMedGoogle Scholar
  • 20 Meachim G. The effect of scarification on articular cartilage in the rabbit. J Bone Joint Surg Am 2001; 45: 150-161.
    PubMedGoogle Scholar