Human Platelet CD36 (GPIIIb, GPIV) Binds to Cholesteryl-Hemisuccinate and Can Be Purified by a Simple Two-Step Method Making Use of this Property

A. Kronenberg; H. Grahl; B. Kehrel

doi:10.1055/s-0037-1615113

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1998; 79(05): 1021-1024
DOI: 10.1055/s-0037-1615113

Review Article

Schattauer GmbH

Human Platelet CD36 (GPIIIb, GPIV) Binds to Cholesteryl-Hemisuccinate and Can Be Purified by a Simple Two-Step Method Making Use of this Property

A. Kronenberg

¹From the Department of Internal Medicine, University of Münster, Münster, Germany

,

H. Grahl

¹From the Department of Internal Medicine, University of Münster, Münster, Germany

,

B. Kehrel

¹From the Department of Internal Medicine, University of Münster, Münster, Germany

› Author Affiliations

Abstract

Summary

CD36, also known as GPIIIb or GPIV, is a main protein of the platelet membrane. Accumulating evidence implicates CD36 in the pathogenesis of atherosclerosis. CD36 binds to cholesteryl-hemisuccinate coupled to agarose. This binding may be of physiologic relevance since CD36 has a long extracellular hydrophobic region at its N-terminus and belongs to a family of proteins involved in lipid metabolism. This newly discovered binding property of CD36 was used to develop a rapid, efficient and gentle two-step purification method for CD36 from human platelets.

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References

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