Smooth Muscle Cell Migration Promoting Activity of Plasma Predicts Restenosis in Patients with Peripheral Arterial Occlusive Disease Undergoing Angioplasty

Regina E. Roller; Sandra Janisch; Erich Kvas; Wolfgang J. Schnedl; Bernd R. Binder; Johann Wojta; Christian Korninger

doi:10.1055/s-0037-1614179

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2000; 84(06): 1113-1116
DOI: 10.1055/s-0037-1614179

Review Article

Schattauer GmbH

Smooth Muscle Cell Migration Promoting Activity of Plasma Predicts Restenosis in Patients with Peripheral Arterial Occlusive Disease Undergoing Angioplasty

Regina E. Roller

¹From the Departments of Vascular Biology and Thrombosis Research, University of Vienna, Austria

²Internal Medicine, Karl Franzens University School of Medicine, Graz, Austria

,

Sandra Janisch

¹From the Departments of Vascular Biology and Thrombosis Research, University of Vienna, Austria

,

Erich Kvas

³Medical Physiology, University of Graz, Austria

,

Wolfgang J. Schnedl

²Internal Medicine, Karl Franzens University School of Medicine, Graz, Austria

,

Bernd R. Binder

¹From the Departments of Vascular Biology and Thrombosis Research, University of Vienna, Austria

,

Johann Wojta

¹From the Departments of Vascular Biology and Thrombosis Research, University of Vienna, Austria

⁵Cardiology, General Hospital Vienna, Austria

,

Christian Korninger

⁴Hematology, General Hospital Vienna, Austria

› Author Affiliations

Abstract

Summary

Background

Efficacy of percutaneous transluminal angioplasty (PTA) is limited by restenosis occurring in a large proportion of patients. Smooth muscle cell (SMC) migration is a major pathomechanism of restenosis. We studied SMC migration inducing activity of plasma from patients with peripheral arterial occlusive disease (PAOD) undergoing PTA.

Methods and Results

SMC migration was determined in a two-dimensional assay system after addition of 1/25 plasma dilutions. Mean increase in migration area was 65.5 ± 33.8% in normal controls and 67.7 ± 53.2% in patients with PAOD. 6 hours after PTA, plasmatic migration inducing activity was largely unchanged. In 19/30 patients with restenosis (6 months after PTA) migration promoting activity (82.7 ± 60.0) was significantly higher than in 11/30 patients with patent vessels (41.8 ± 21.0; p = 0.03). No correlation of clinical risk factors with outcome was observed. A weak correlation was found between plasmatic migration promoting activity and levels of epidermal growth factor and transforming growth factor-β.

Conclusion

The capacity of human plasma to stimulate SMC migration in tissue culture can be used to assess the risk for restenosis after PTA in patients with PAOD.

Key words

Peripheral arterial occlusive disease - angioplasty - restenosis - smooth muscle cells - growth factors

Full Text

References

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