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Thromb Haemost 2000; 84(06): 1023-1030
DOI: 10.1055/s-0037-1614166
Review Article
Schattauer GmbH

Early Embryonic Expression of Murine Coagulation System Components

Kelly Ong
1   From the Institute of Medical Sciences, Toronto, Ontario, Canada
,
Wendy Horsfall
1   From the Institute of Medical Sciences, Toronto, Ontario, Canada
2   The Departments of Medicine, Toronto, Ontario, Canada
,
Edward M. Conway
1   From the Institute of Medical Sciences, Toronto, Ontario, Canada
2   The Departments of Medicine, Toronto, Ontario, Canada
3   Medical Biophysics, Toronto, Ontario, Canada
4   University of Toronto, and the Division of Hematology/Oncology, The Toronto Hospital, Toronto, Ontario, Canada
5   Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, University of Leuven, Leuven, Belgium
,
Andre C. Schuh
1   From the Institute of Medical Sciences, Toronto, Ontario, Canada
2   The Departments of Medicine, Toronto, Ontario, Canada
3   Medical Biophysics, Toronto, Ontario, Canada
4   University of Toronto, and the Division of Hematology/Oncology, The Toronto Hospital, Toronto, Ontario, Canada
› Author AffiliationsThe authors thank Xiang-Fu Wu for technical assistance, Daniel Dumont for helpful discussions, and Norman Lassam for reviewing the manuscript. This work was supported by grants from The Heart and Stroke Foundation of Ontario, Canada (EMC) and the Medical Research Council of Canada (ACS).
Further Information

Publication History

Received 17 December 1999

Accepted after resubmission 01 June 2000

Publication Date:
13 December 2017 (online)

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Summary

The development of the embryonic coagulation system, and its contribution to the maintenance of vascular integrity during the formation of embryonic blood vessels, remain poorly understood. We have characterized the temporal expression patterns of 27 hemostasis-related genes during murine development. We show that, although most coagulation and fibrinolysis-related factors are expressed coordinately b 7.5 dpc, several, including FIX, FXII and PAI2, are not detectable until later developmental timepoints. The expression of hemostasis-specific genes prior to the formation of a functional circulatory system supports the view that some coagulation factors have additional non-hemostatic functions during development. In addition, the discordant expression of some factors suggests that the embryonic hemostatic system may be distinct from that of the adult. These analyses will help to elucidate the regulation of hemostasis during embryonic/vascular development, and will provide a framework to facilitate the interpretation of gene inactivation studies.

Keywords

Embryo - hemostasis - vascular development - knock-out
 

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