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Thromb Haemost 2000; 83(01): 141-147
DOI: 10.1055/s-0037-1613770
Commentary
Schattauer GmbH

Nucleotide Sequence of the Gene Encoding Murine Tissue Factor Pathway Inhibitor-2

Yoshiaki Kazama
1   From the Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA
,
Shintaro Kamei
1   From the Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA
,
Joseph L. Kuijper
2   ZymoGenetics, Inc., Seattle, WA, USA
,
Donald C. Foster
2   ZymoGenetics, Inc., Seattle, WA, USA
,
Walter Kisiel
1   From the Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA
› Author Affiliations
Further Information

Publication History

Received 23 August 1999

Accepted after revision 28 September 1999

Publication Date:
06 December 2017 (online)

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Summary

Tissue factor pathway inhibitor-2 (TFPI-2), also known as placental protein 5, is a 32 kDa extracellular matrix-associated serine proteinase inhibitor consisting of three tandemly-arranged Kunitz-type domains. Two overlapping genomic clones containing sequences encoding murine TFPI-2 were isolated from a A FIXII 129 SVJ mouse genomic library, and the complete nucleotide sequence of the gene was determined. The murine TFPI-2 gene spans approximately 9.3 kilobases and consists of five exons and four introns. The nucleotide sequences surrounding all the exon-intron boundaries are highly conserved and obey the GT-AG rule. Each Kunitz-type domain is encoded by a single exon, similar to that observed for other Kunitz-type proteinase inhibitors. A total of 1,577 bp of the 3’-flanking region contains a probable polyadenylation site (ATTAAA) at +5,759 and an apparent cleavage or termination site (CATTG) at +6,170. The 5’-flanking region of the murine TFPI-2 gene contains a prototypical TATA box, a GC box and two CAAT boxes. In addition, several candidate transcription factor binding sites responsible for placenta-, endothelial cell-, and smooth muscle cell-specific expression of the TFPI-2 gene were also identified.

Keywords

Tissue factor pathway inhibitor-2 - Kunitz-type proteinase inhibitor - gene - mouse
 

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