Synfacts 2018; 14(02): 0113
DOI: 10.1055/s-0037-1609195
Synthesis of Natural Products and Potential Drugs
© Georg Thieme Verlag Stuttgart · New York

Synthesis of an Inhibitor of Histone Lysine Demethylases KDM2/7

Philip Kocienski
Brennan PE. * Smith MD. * et al. University of Oxford, UK and University of Toronto, Canada
Discovery of a Highly Selective Cell-Active Inhibitor of the Histone Lysine Demethylases KDM2/7.

Angew. Chem. Int. Ed. 2017;
56: 15555-15559
Further Information

Publication History

Publication Date:
18 January 2018 (online)



The target molecule M is a first-in-class highly selective cell-active inhibitor of the histone lysine demethylases KDM2/7 that are involved in epigenetic gene expression. In total 45 racemic N-acyl indoline derivatives were prepared, from which the enantiomers of M were selected for further evaluation.



Synthesis of the indoline (S,S)-H entails treatment of the N-aryl imine F with cesium hydroxide under phase-transfer conditions in the presence of the quinine-derived salt G (0.1 equiv). A delocalized 2-aza-pentadienyl anion is generated that undergoes 6π electrocyclization in 89% yield (dr = 10:1, er = 88:12). Preparative chiral HPLC then delivered H with dr > 20:1 and er > 99:1. For details on the asymmetric electrocyclization, see: E. E. Maciver, S. Thompson, M. D. Smith ­Angew. Chem. Int. Ed. 2009, 48, 9979).