Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596899
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Investigation of in vitro antifungal efficacy of selected extracts of Greek natural products on Malassezia sps

Authors

  • A Velegraki

    1   Div. of Microbiology Faculty of Medicine, Athens, Greece

  • K Graikou

    2   Div. of Pharmacognosy & Chemistry of Natural Products, School of Pharmacy, National and Kapodistrian University of Athens, Zografou 157 71 Athens, Greece

  • S Kritikou

    1   Div. of Microbiology Faculty of Medicine, Athens, Greece

  • M Varsani

    2   Div. of Pharmacognosy & Chemistry of Natural Products, School of Pharmacy, National and Kapodistrian University of Athens, Zografou 157 71 Athens, Greece

  • I Chinou

    2   Div. of Pharmacognosy & Chemistry of Natural Products, School of Pharmacy, National and Kapodistrian University of Athens, Zografou 157 71 Athens, Greece
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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Malassezia sps. are lipophilic yeasts which belong to the resident flora of human skin and has been responsible for the pathogenesis of several skin diseases such as dandruff, pityriasis versicolor, folliculitis, seborrhoeic dermatitis and some forms of atopic dermatitis. The synthetic drugs available were proved to be toxic and expensive when used in prolonged treatment [1].

The present study was aimed to evaluate the inhibitory effects of selected Greek plants from Crete Island as well as ethanolic extracts from selected Greek (Peloponnese, Macedonia and Crete) propolis samples against reference and clinical Malassezia strains [2].

The water extracts of Origanum dictamnus L. (Lamiaceae) and Cistus creticus L. (Cistaceae), which are native and endemic to the island of Crete traditionally used in Greece, were studied for their bioactivities against a panel of Malassezia strains.

A total of 18 type, reference and clinical Malassezia strains (M. furfur, M. restricta and M. globosa) were tested. All compound dilutions were made in DMSO. Subsequent serial dilutions of each compound were performed as described before [2].

The Cistus creticus decoction showed strong bio-activity against the major dandruff agents (M. restricta and M. globosa) at clinically significant concentrations (MIC 4 – 16 mg/L), while the other tested samples demonstrated limited to no activity against clinical strains. It is noteworthy that the antifungal activity of the decoction of Cistus creticus was comparable to the activity of the known antibiotic amphotericin B and could be considered as a source for developing potentially better anti-malassezial agents.

Keywords: Origanum dictamnus, Cistus creticus, Greek propolis, Malassezia.

References:

[1] Sreelatha GL, Babu UV, Sharath Kumar LM, Soumya K, Sharmila T. Investigation on biochemical characterisation and in vitro antifungal efficacy of plant extracts on Malassezia furfur. Int J Pharm Bio Scie 2015; 6: B1027-B1041

[2] Velegraki A, Alexopoulos EC, Kritikou S, Gaitanis G. Use of fatty acid RPMI 1640 media for testing susceptibilities of eight Malassezia species to the new triazole posaconazole and six established antifungal agents by a modified NCCLS M27-A2 microdilution method and Etest. J Clin Microbiol 2004; 42: 3589 – 3593


No conflict of interest has been declared by the author(s).