Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596707
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Inhibitory effect of isolated constituents from sclerotia of Poria cocos on LPS-induced NO production

SR Lee
1  School of Pharmacy, Sungkyunkwan University, Suwon 440 – 746, Republic of Korea
,
S Lee
1  School of Pharmacy, Sungkyunkwan University, Suwon 440 – 746, Republic of Korea
,
HJ Eom
1  School of Pharmacy, Sungkyunkwan University, Suwon 440 – 746, Republic of Korea
,
HR Kang
1  School of Pharmacy, Sungkyunkwan University, Suwon 440 – 746, Republic of Korea
,
JS Yu
1  School of Pharmacy, Sungkyunkwan University, Suwon 440 – 746, Republic of Korea
,
TK Lee
1  School of Pharmacy, Sungkyunkwan University, Suwon 440 – 746, Republic of Korea
,
J Baek
1  School of Pharmacy, Sungkyunkwan University, Suwon 440 – 746, Republic of Korea
,
D Lee
1  School of Pharmacy, Sungkyunkwan University, Suwon 440 – 746, Republic of Korea
,
WS Suh
1  School of Pharmacy, Sungkyunkwan University, Suwon 440 – 746, Republic of Korea
,
KH Kim
1  School of Pharmacy, Sungkyunkwan University, Suwon 440 – 746, Republic of Korea
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 
 

    Poria cocos Wolf, well known as 'Fu-Ling', is a medicinal mushroom of the family Polyporaceae, which is widely distributed in Korea, China, and East Asian countries [1]. This mushroom is widely utilized in traditional medicine for sedative, diuretic, and tonic effects [2]. Recently, the sclerotia of P. cocos showed potential as a natural drug for improving diuresis, treatment of invigorating spleen, and stabilizing the mind [3,4]. Previous phytochemical studies reported that more than 50 triterpenoids were isolated from P. cocos, and most of them were lanostane-type triterpenoids [5]. In our continuing search for bioactive constituents from Korean wild mushrooms, we investigated the EtOH extract of the sclerotia of P. cocos in the course of bioassay-guided isolation. The EtOH extract was solvent-partitioned with n-hexane, CH2Cl2, EtOAc, and n-BuOH. We found that the CH2Cl2 fraction showed significant inhibitory activity on nitric oxide (NO) production in LPS-induced RAW 264.7 macrophage cells. Phytochemical investigation of the CH2Cl2 fraction led to the isolation of six triterpenoids (1 – 6), one lignan (7), and one phenolic compound (8). All the isolated compounds were structurally identified by spectroscopic methods (1H-NMR, 13C-NMR, MS) and comparison with previously reported spectroscopic data. Anti-inflammatory activities of the isolated compounds were determined as potential regulators for excessive inflammatory response in RAW 264.7 macrophage cells.

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    Acknowledgements: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2015R1C1A1A02037383).

    Keywords: Poria cocos, Polyporaceae, triterpenoid, nitric oxide.

    References:

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    No conflict of interest has been declared by the author(s).

     
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