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Inhibitory effect of isolated constituents from sclerotia of Poria cocos on LPS-induced NO production
14 December 2016 (online)
Poria cocos Wolf, well known as 'Fu-Ling', is a medicinal mushroom of the family Polyporaceae, which is widely distributed in Korea, China, and East Asian countries . This mushroom is widely utilized in traditional medicine for sedative, diuretic, and tonic effects . Recently, the sclerotia of P. cocos showed potential as a natural drug for improving diuresis, treatment of invigorating spleen, and stabilizing the mind [3,4]. Previous phytochemical studies reported that more than 50 triterpenoids were isolated from P. cocos, and most of them were lanostane-type triterpenoids . In our continuing search for bioactive constituents from Korean wild mushrooms, we investigated the EtOH extract of the sclerotia of P. cocos in the course of bioassay-guided isolation. The EtOH extract was solvent-partitioned with n-hexane, CH2Cl2, EtOAc, and n-BuOH. We found that the CH2Cl2 fraction showed significant inhibitory activity on nitric oxide (NO) production in LPS-induced RAW 264.7 macrophage cells. Phytochemical investigation of the CH2Cl2 fraction led to the isolation of six triterpenoids (1 – 6), one lignan (7), and one phenolic compound (8). All the isolated compounds were structurally identified by spectroscopic methods (1H-NMR, 13C-NMR, MS) and comparison with previously reported spectroscopic data. Anti-inflammatory activities of the isolated compounds were determined as potential regulators for excessive inflammatory response in RAW 264.7 macrophage cells.
Acknowledgements: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2015R1C1A1A02037383).
Keywords: Poria cocos, Polyporaceae, triterpenoid, nitric oxide.
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No conflict of interest has been declared by the author(s).