Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596702
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Bioactive type A proanthocyanins from fungus Laurobasidium lauri

Authors

  • J Serina

    1   Centro de Química da Madeira, Faculty of Exact Sciences and Engineering, Madeira University, Campus Penteada, 9000 – 390, Funchal, Portugal

  • MJ Carvalho

    2   Faculty of Exact Sciences and Engineering, Madeira University, Campus Penteada, 9000 – 390, Funchal, Portugal

  • T Weinhold

    1   Centro de Química da Madeira, Faculty of Exact Sciences and Engineering, Madeira University, Campus Penteada, 9000 – 390, Funchal, Portugal

  • PC Castilho

    1   Centro de Química da Madeira, Faculty of Exact Sciences and Engineering, Madeira University, Campus Penteada, 9000 – 390, Funchal, Portugal
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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Laurobasidium lauri, commonly known as Madre de Louro, is a parasitic fungus of Laurus trees which has a large use in local folk medicine, usually as an alcoholic tincture [1]. In this study, the antioxidant and antimicrobial activities of several extracts of Madre de Louro were evaluated and enzymatic studies were performed using in-vitro assays [2]. The ethanol extract revealed higher activity in all assays. Results also showed the ethanol extract to be a potent inhibitor of α-glucosidase, compared to positive control acarbose. IC50 values were 1.04 µg/mL for the methanol extract and IC50 3.43 µg/mL for acarbose. Alcoholic extracts exert antibacterial activity against Staphylococcus aureus, with a MIC of 62.5 µg/mL. Thus, a LC-MSn technique was applied to determine the main compounds in this extract, revealing catechin monomers, dimers and trimers, with special relevance for A-type procyanidins. Molecular docking was performed in an attempt to identify the compounds which exerted the observed inhibitory effects. However, per se, none of the analysed compounds had a binding affinity close to acarbose (-15.6 kcal/mol) in the same docking conditions, but a combination of two ligands leading to synergistic interactions is proposed as responsible for the enhanced activity observed in vitro.

Acknowledgements: The Portuguese Foundation for Science and Technology (FCT) is acknowledged for the strategic project PEst-OE/QUI-674/UI0674/2014.

Keywords: Laurobasidium lauri, procyanidins, LC-MSn, α-glucosidase, molecular docking.

References:

[1] Tavares L, Carrilho D, Tyagi M, Barata D, Serra AT, Duarte CMM, Duarte RO, Feliciano RP, Bronze MR, Chicau P, Espírito-Santo MD, Ferreira RB, dos Santos CN. Antioxidant capacity of Macaronesian traditional medicinal plants. Molecules 2010; 15: 2576 – 2592

[2] Gouveia-Figueira SC, Gouveia CA, Carvalho MJ, Rodrigues AI, Nording ML, Castilho PC. Antioxidant capacity, cytotoxicity and antimycobacterial activity of Madeira archipelago endemic Helichrysum dietary and medicinal plants. Antioxidants 2014; 3: 713 – 729


No conflict of interest has been declared by the author(s).