Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596499
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Chrysophanol- and nepodin-8-O-β-D-glucopyranoside from Rumex acetosella, the cytotoxicity towards drug sensitive and multi- drug resistant T leukaemia cancer cells

Authors

  • N Ozenver

    1   Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Sihhiye, 06100, Ankara, Turkey
    2   Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany

  • M Saeed

    2   Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany

  • Z Guvenalp

    3   Ataturk University, Faculty of Pharmacy, Department of Pharmacognosy, 25240, Erzurum, Turkey

  • LO Demirezer

    1   Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Sihhiye, 06100, Ankara, Turkey

  • T Efferth

    2   Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany
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Publikationsverlauf

Publikationsdatum:
14. Dezember 2016 (online)

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    Rumex acetosella L. (Polygonaceae) is traditionally used in cancer treatment by few tribes in Canada [1]. It contains anthraquinones [2], flavonoids [2, 3] and other phenolics [4]. We isolated anthraquinone and naphtalene glycosides by using different column chromatography techniques and determined their structures with NMR spectroscopy as chrysophanol-8-O-β-D glucopyranoside and nepodin-8-O-β-D-glucopyranoside. Anthraquinones inhibit tumor growth on some cancer cells. Additionally naphtalene derivatives are shown to inhibit tumor growth at nM ranges on few cell lines. Multidrug resistance in cancer represents a major problem in chemotherapy. Natural compounds having fewer side effects can be proper candidates for cancer treatment. Therefore potential drug candidates from natural sources need to be searched. In this study we assessed the cytotoxicity of an anthraquinone and a naphthalene derivative from the roots of R. acetosella against drug sensitive CCRF-CEM and multidrug resistant P- glycoprotein-over-expressing CEM/ADR5000 leukemia cells. The resazurin assay was used to determine the cytotoxicity of the compounds. The recorded IC50 values for chrysophanol-8-O- β-D-glucopyranoside and nepodin-8-O- β-D-glucopyranoside ranged from 69.05µM to 74.9µM (towards leukemia CCRF-CEM cells) and from 104.52µM to 82.9µM (towards leukemia CEM/ADR5000 cells), compared to established chemotherapeutic agent “doxorubicin” which showed 0.0007µM (towards leukemia CCRF-CEM cells) and 48.9µM towards leukemia CEM/ADR5000 cells. Even if the compounds didn't show remarkable cytotoxicity on leukemia cells, further studies need to be conducted on natural compounds that could have a potential to combat with drug resistance in cancer.

    Acknowledgments: This study was supported by The Scientific and Technological Research Council of Turkey (TÜBITAK) 2214-A scholarship.

    Keywords: Anthraquinone, naphtalene, cytotoxicity, Resazurin reduction assay, mutidrug resistantance (MDR), cancer.

    References:

    [1] Leonard SS, Keil D, Mehlman T, Proper S, Shi XL, Harris GK. Essiac tea: Scavenging of reactive oxygen species and effects on DNA damage. J Ethnopharmacol 2006; 103: 288 – 296

    [2] Duke JA. Handbook of Biological Active Phytochemicals & Their Activity: CRC-Press; 1992.

    [3] Thomas R. The Essiac Report: The True Story of a Canadian Herbal Cancer Remedy and of the Thousands of Lives it Continues to Save: Alternative Treatment Information Network; 1993

    [4] Đurðević L, Gajić G, Jarić S, Kostić O, Mitrović M, Pavlović P. Analysis of benzoic and cinnamic acid derivatives of some medicinal plants in Serbia. Arch Biol Sci 2013; 65: 603 – 609


     

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