Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596479
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Phenol and flavonoid contents and in vitro antibacterial activity of Eryngium Pristis Cham. & Schltdl. (Apiaceae) against methicillin-resistant Staphylococcus aureus (MRSA)

LS Fernandes
1   Pharmacy Course, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
DM Pedrosa
1   Pharmacy Course, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
C Feres-Netto
1   Pharmacy Course, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
YFG Costa
1   Pharmacy Course, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
ALP Ferreira
2   Laboratory of Bacteriology, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Av. Professor Rodolpho Paulo Rocco, Ilha do Fundão, CEP 20380 – 010, Rio de Janeiro, Brazil
,
G Del-Vecchio-Vieira
3   Laboratory of Pharmacology of Natural Products, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
OV Sousa
4   Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
MS Alves
4   Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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    Eryngium pristis (Apiaceae) is a South America's native shrub poorly scientific explored [1]. This medicinal plant has several secondary metabolites such as phenolic substances, mainly flavonoids, which have been studied as potent antibacterial agents [2]. The current study aimed to determine the total phenolic and flavonoids contents and the antibacterial activity of ethanolic extract (EE) and hexane (HF), dichloromethane (DF), ethyl acetate (AF) and butanol (BF) fractions of E. pristis leaves. Total phenolic and flavonoids were determined by Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. The antibacterial activity was established by the Minimal Inhibitory Concentration (MIC) using microdilution method [3] followed by the Minimal Bactericidal Concentration (MBC), which allows to classify the effect as bacteriostatic or bactericidal [4]. Staphylococcus aureus subsp. aureus (ATCC®29213™ and ATCC®6538™) were used as reference strains and methicillin-resistant Staphylococcus aureus (MRSA 1485279/1605677/1664534/1688441/1830466) as clinical strains. The total phenolic content varied from 5.05 to 20.10 g/100 g, while total flavonoids, from 4 to 9.37 g/100 g. EE (bactericidal) and HF and AF (bacteriostatic) were active against S. aureus ATCC®29213™ with MIC of 2.5, 2.5 and 5 mg/mL, respectively. HF (bacteriostatic) and EE and AF (bactericidal) were active against S. aureus ATCC®6538™ with MIC of 5, 1.25 and 2.5 mg/mL, respectively. EE, HF and AF (bacteriostatic) produced MIC of 5, 5 and 2.5 mg/mL, in this order, against MRSA 1485279 and 1688441. EE, HF, DF, AF and BF (bacteriostatic) were active against MRSA 1605677 with MIC of 2.5, 5, 5, 2.5 and 5 mg/mL, respectively. EE [bacteriostatic (1664534) and bactericidal (1830466)], HF, DF and AF (bacteriostatic) produced MIC of 2.5, 2.5, 5 and 1.25 mg/mL, in this order, against MRSA 1664534 and 1830466. These results suggest that E. pristis is an interesting source of active constituents, leading to prospect of new therapeutic approaches against S. aureus infections.

    Acknowledgements: This investigation was financially supported by UFJF, FAPEMIG, CNPq and CAPES.

    Keywords: Eryngium pristis, Apiaceae, Flavonoids, Anti-Bacterial Agents, Medicinal Plants.

    References:

    [1] Souza HC, Campos CCF, Borém RAT, Castro DM. Aspectos da polinização de Eryngium pristis Cham. & Schltdl. na reserva de cerrado stricto sensu da UFLA-MG. Anais do VIII Congresso de Ecologia do Brasil, Caxambu, 2007


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    No conflict of interest has been declared by the author(s).