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Comparative analysis of combinatory effects of organic extracts from Cleome spinosa Jaqc and oxacilin against Staphylococcus aureus
14 December 2016 (online)
Despite all efforts, there is a clear and urgent need for developing alternative strategies to overcome bacterial drug resistance . Combination therapy has been pointed as one approach for overcoming the problem of antibiotic resistance, and natural products has been reported as potent modulators of drugs action . Cleome spinosa Jacq. (Cleomaceae) is a medicinal plant widely used to treat inflammatory and infectious processes in the Northeast region of Brazil . Our previous results showed that its extracts have a broad spectrum of antimicrobial activities, specially chloroform and cyclehexane extracts against Staphylococcus aureus. This work evaluated the combinatory effects of extracts (chloroform or cyclehexane) from the roots and leaves of C. spinosa against clinical isolates of S. aureus. The anti-S. aureus action was evaluated by determination of the minimum inhibitory concentration (MIC) against eight strains with different antibiotic-resistance profiles. Combinatory effects with oxalicin were assessed by Fractional Inhibitory Concentration (ΣFIC) . The phenolic and flavonoid contents were also determined. The extracts were active against all strains, but the chloroform ones showed the best (p < 0.05), with MIC50 values (concentration able to inhibit 50% of the strains) of 0.78 mg/mL (leaves) and 3.12 mg/mL (roots). These extracts showed the highest phenolic and flavonoid contents, which have strong correlations (ρ > -0.87) with MIC values found. The extracts from chloroform also showed the best combinatory effects, with synergistic action with oxacilin against all strains (at least at one combined proportion). Cyclohexane extracts showed synergistic action against 70% of strains. Our results showed that chloroform and cyclohexane extracts from leaves and roots of C. spinosa are able to increase the action of oxacilin. Thus, these extracts are source of promising natural products for the development of new anti-S. aureus formulations.
Acknowledgements: Program Science without borders CAPES/Brazil.
Keywords: Caatinga natural products, drug resistance, synergistic action.
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No conflict of interest has been declared by the author(s).