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Planta Med 2015; 81(17): 1582-1591
DOI: 10.1055/s-0035-1546246
Mini Reviews
Georg Thieme Verlag KG Stuttgart · New York

Solvent System Selection Strategies in Countercurrent Separation

Yang Liu
1   Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, United States
,
J. Brent Friesen
1   Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, United States
2   Physical Sciences Department, Rosary College of Arts and Sciences, Dominican University, River Forest, Illinois, United States
,
James B. McAlpine
1   Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, United States
3   Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, United States
,
Guido F. Pauli
1   Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, United States
3   Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, United States
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Weitere Informationen

Publikationsverlauf

received 04. Oktober 2014
revised 09. März 2015

accepted 03. Juni 2015

Publikationsdatum:
21. September 2015 (online)

    Lizenzen und Reprints

Abstract

The majority of applications in countercurrent and centrifugal partition chromatography, collectively known as countercurrent separation, are dedicated to medicinal plant and natural product research. In countercurrent separation, the selection of the appropriate solvent system is of utmost importance as it is the equivalent to the simultaneous choice of column and eluent in liquid chromatography. However, solvent system selection is often laborious, involving extensive partition and/or analytical trials. Therefore, simplified solvent system selection strategies that predict the partition coefficients and, thus, analyte behavior are in high demand and may advance both the science of countercurrent separation and its applications. The last decade of solvent system selection theory and applications are critically reviewed, and strategies are classified according to their data input requirements. This offers the practitioner an up-to-date overview of rationales and methods for choosing an efficient solvent system, provides a perspective regarding their accuracy, reliability, and practicality, and discusses the possibility of combining multiple methods for enhanced prediction power.

Key words

countercurrent separation - countercurrent chromatography - centrifugal partition chromatography - solvent system selection

Supporting Information

 

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