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J Knee Surg 2016; 29(01): 047-062
DOI: 10.1055/s-0034-1394165
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Identification of Novel Synovial Fluid Biomarkers Associated with Meniscal Pathology

Brandon L. Roller
1   Arthrex, Inc., Naples, Florida
2   Comparative Orthopaedic Laboratory, University of Missouri, Columbia, Missouri
,
Farrah Monibi
2   Comparative Orthopaedic Laboratory, University of Missouri, Columbia, Missouri
,
Aaron M. Stoker
2   Comparative Orthopaedic Laboratory, University of Missouri, Columbia, Missouri
,
B. Sonny Bal
3   Department of Orthopaedic Surgery, University of Missouri, Columbia, Missouri
,
James L. Cook
2   Comparative Orthopaedic Laboratory, University of Missouri, Columbia, Missouri
3   Department of Orthopaedic Surgery, University of Missouri, Columbia, Missouri
› Author Affiliations
Further Information

Publication History

08 April 2014

01 September 2014

Publication Date:
22 October 2014 (online)

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Abstract

The menisci are integral components within the knee for ensuring optimal joint function. The overall goal of this study was to identify proteomic markers of meniscal disease within synovial fluid samples obtained from control knees versus knees affected with varying degrees of meniscal injury and osteoarthritis. Joint fluid samples were collected before the patient underwent an arthroscopic knee procedure or total knee arthroplasty. Normal controls included patients younger than 30 years with no history of anterior cruciate ligament, posterior cruciate ligament, or meniscal injury. A total of 21 joint fluid aspirates were analyzed using mass spectrometry, and a total of 296 proteins were identified. Among these, 50 proteins were determined to be of interest as potential biomarkers based on initial analysis and known functions in articular metabolism. Further statistical analysis comparing protein concentrations among clinical groups identified 13 proteins with significant differences between at least two of the patient cohorts. These data provide novel information for the investigation of synovial fluid biomarkers and treatment strategies for meniscal pathology.

Keywords

synovial fluid - biomarkers - proteomics - knee - pathology
 

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