Changes of Number and Function of Late Endothelial Progenitor Cells in Peripheral Blood of COPD Patients Combined with Pulmonary Hypertension

Pei Liu; Hongmei Zhang; Jianxin Liu; Chunfeng Sheng; Linlin Zhang; Yanjun Zeng

doi:10.1055/s-0034-1389261

The Thoracic and Cardiovascular Surgeon, Table of Contents

Thorac Cardiovasc Surg 2016; 64(04): 323-329
DOI: 10.1055/s-0034-1389261

Original Basic Science

Georg Thieme Verlag KG Stuttgart · New York

Changes of Number and Function of Late Endothelial Progenitor Cells in Peripheral Blood of COPD Patients Combined with Pulmonary Hypertension

Authors

Pei Liu

¹Department of Intensive Care Unit, Taihe Hospital of Hubei Medical University, Shiyan, Hubei, China
Hongmei Zhang

²Department of Respiratory Diseases, Taihe Hospital of Hubei Medical University, Shiyan, Hubei, China
Jianxin Liu

³Department of Ultrasound Imaging, Taihe Hospital of Hubei Medical University, Shiyan, Hubei, China
Chunfeng Sheng

¹Department of Intensive Care Unit, Taihe Hospital of Hubei Medical University, Shiyan, Hubei, China
Linlin Zhang

⁴Department of Engineering Mechanics, Beijing University of Technology, Beijing, China
Yanjun Zeng

⁴Department of Engineering Mechanics, Beijing University of Technology, Beijing, China

Abstract

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Abstract

Objective The objective of this study was to investigate the changes of number and function of late endothelial progenitor cells (EPCs) in peripheral blood of chronic obstructive pulmonary disease (COPD) patients combined with pulmonary hypertension.

Subjects and Methods The study enrolled 120 cases including 40 non-COPD and pulmonary arterial hypertension (PAH) patients (non-COPD group), 40 COPD non-PAH patients (COPD group), and 40 COPD patients combined with PAH (COPD + PAH group). Peripheral blood mononuclear cells were separated by density gradient centrifugation, cultured for 21 days, and then identified as late endothelial progenitor cells. The cell colonies were counted. MTT assay, modified Boyden chamber assay, and human fibronectin plates were used to measure the proliferation, migration, and adhesion functions of the late endothelial progenitor cells, respectively.

Results Compared with non-COPD and COPD groups, the number of peripheral blood late EPCs in COPD + PAH group was significantly reduced, and the proliferation, adhesion, and migration capacities were significantly lowered; the differences were statistically significant (p < 0.05). The number and function of late EPCs decreased with the increase of pulmonary artery pressure (p < 0.05).

Conclusion The number of late EPCs in COPD patients combined with pulmonary hypertension was reduced, which implies the impaired cell functions. The changes of number and function were negatively correlated with the severity of pulmonary hypertension.

Keywords

chronic obstructive pulmonary disease - pulmonary hypertension - late EPCs

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References

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