Comparison of Body Composition Techniques before and after a 161-Km Ultramarathon Using DXA, BIS and BIA

Abstract

The low cost, ease of application and portability of bioelectrical impedance analysis (BIA) and spectroscopy (BIS) devices make them attractive tools for measuring acute changes in body composition before and after exercise, despite potential limitations from active compartmental fluid shifts. The primary study aim was to evaluate use of dual energy x-ray absorptiometry (DXA) against BIA and BIS in measurements of percent body fat (%BF) and percent total body water (%TBW) before and after prolonged endurance exercise. 10 runners were measured pre-race and at race finish. Significant linear relationships were noted pre-race between DXA vs. BIS for %BF (r²=0.76; p<0.01) and %TBW (r²=0.74; p<0.01). Significant correlations were noted at race finish between DXA vs. BIS for %BF (r²=0.64; p<0.01) and %TBW (r²=0.66; p<0.05), but only when one outlier was removed. Limits of agreement (LOA) between DXA vs. BIS were wide for both %BF (mean difference of −3.6, LOA between 5.4 and −12.6) and %TBW (mean difference 2.4, LOA between 0.4 and −4.6). LOA was closer between the DXA vs. BIA with DXA measuring slightly higher than BIA for %BF (mean difference of 0.5, LOA between 2.1 and −3.1) and slightly lower than BIA for %TBW (mean difference 0.3, LOA between 3.3 and −2.7). Linear correlations between DXA vs. BIA were not statistically significant for %BF or %TBW before or after the race. DXA measurement of acute changes in %BF and %TBW are not congruent with BIA or BIS measurements. These 3 techniques should not be utilized interchangeably after prolonged endurance running.

Key words

body composition - ultramarathon - DXA Scan

• References

• 1 Adams CS, Korytko AI, Blank JL. A novel mechanism of body mass regulation. J Exp Biol 2001; 204: 1729-1734
• 2 Armstrong LE, Kenefick RW, Castellani JW, Riebe D, Kavouras SA, Kuznicki JT, Maresh CM. Bioimpedance spectroscopy technique: intra-, extracellular, and total body water. Med Sci Sports Exerc 1997; 29: 1657-1663
• 3 Baker LB, Lang JA, Kenney WL. Change in body mass accurately and reliably predicts change in body water after endurance exercise. Eur J Appl Physiol 2009; 105: 959-967
• 4 Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986; 1: 307-310
• 5 Bolotin HH, Sievanen H, Grashuis JL, Kuiper JW, Jarvinen TL. Inaccuracies inherent in patient-specific dual-energy X-ray absorptiometry bone mineral density measurements: comprehensive phantom-based evaluation. J Bone Miner Res 2001; 16: 417-426
• 6 Carlsson E, Bosaeus I, Nordgren S. Body composition in patients with short bowel syndrome: an assessment by bioelectric impedance spectroscopy (BIS) and dual-energy absorptiometry (DXA). Eur J Clin Nutr 2004; 58: 853-859
• 7 Chorley J, Cianca J, Divine J. Risk factors for exercise-associated hyponatremia in non-elite marathon runners. Clin J Sport Med 2007; 17: 471-477
• 8 Cuddy J, Slivka D, Hailes W, Dumke C. Total energy expenditure, body water turnover, hydration status, and blood composition during the Western States 100. Med Sci Sports Exerc 2009; 41: S336
• 9 Deurenberg-Yap M, Schmidt G, van Staveren WA, Hautvast JG, Deurenberg P. Body fat measurement among Singaporean Chinese, Malays and Indians: a comparative study using a four-compartment model and different two-compartment models. Br J Nutr 2001; 85: 491-498
• 10 Fellmann N, Ritz P, Ribeyre J, Beaufrere B, Delaitre M, Coudert J. Intracellular hyperhydration induced by a 7-day endurance race. Eur J Appl Physiol 1999; 80: 353-359
• 11 Going SB, Massett MP, Hall MC, Bare LA, Root PA, Williams DP, Lohman TG. Detection of small changes in body composition by dual-energy x-ray absorptiometry. Am J Clin Nutr 1993; 57: 845-850
• 12 Haderslev KV, Staun M. Comparison of dual-energy X-ray absorptiometry to four other methods to determine body composition in underweight patients with chronic gastrointestinal disease. Metabolism 2000; 49: 360-366
• 13 Hankiss DJ, Atkinson G. Ethical Standards in Sport and Exercise Science Research: 2014 Update. Int J Sports Med 2013; 34: 1025-1028
• 14 Harris RB. Role of set-point theory in regulation of body weight. FASEB J 1990; 4: 3310-3318
• 15 Hew-Butler TD, Ayus JC, Kipps C, Maughan RJ, Mettler S, Meeuwisse WH, Page AJ, Reid SA, Rehrer NJ, Roberts WO, Rogers IR, Rosner MH, Siegel AJ, Speedy DB, Stuempfle KJ, Verbalis JG, Weschler LB, Wharam P. Statement of the Second International Exercise-Associated Hyponatremia Consensus Development Conference, New Zealand, 2007. Clin J Sport Med 2008; 18: 111-121
• 16 Hoffman MD, Hew-Butler T, Stuempfle KJ. Exercise-associated hyponatremia and hydration status in 161-km ultramarathoners. Med Sci Sports Exerc 2013; 45: 784-791
• 17 Horber FF, Thomi F, Casez JP, Fonteille J, Jaeger P. Impact of hydration status on body composition as measured by dual energy X-ray absorptiometry in normal volunteers and patients on haemodialysis. Br J Radiol 1992; 65: 895-900
• 18 Jaffrin MY, Fenech M, Moreno MV, Kieffer R. Total body water measurement by a modification of the bioimpedance spectroscopy method. Med Biol Eng Comput 2006; 44: 873-882
• 19 King S, Wilson J, Kotsimbos T, Bailey M, Nyulasi I. Body composition assessment in adults with cystic fibrosis: comparison of dual-energy X-ray absorptiometry with skinfolds and bioelectrical impedance analysis. Nutrition 2005; 21: 1087-1094
• 20 Knechtle B, Wirth A, Knechtle P, Rosemann T. Increase of total body water with decrease of body mass while running 100 km nonstop – formation of edema?. Res Q Exerc Sport 2009; 80: 593-603
• 21 Kohrt WM. Body composition by DXA: tried and true?. Med Sci Sports Exerc 1995; 27: 1349-1353
• 22 Laforgia J, Dollman J, Dale MJ, Withers RT, Hill AM. Validation of DXA body composition estimates in obese men and women. Obesity (Silver Spring) 2009; 17: 821-826
• 23 Laskey MA. Dual-energy X-ray absorptiometry and body composition. Nutrition 1996; 12: 45-51
• 24 Lebus DK, Casazza GA, Hoffman MD, Ganong AC, Van Loan M. Can changes in body mass and total body water accurately predict hyponatremia following a 161-km running race?. Clin J Sport Med 2010; 20: 193-199
• 25 Maughan RJ, Shirreffs SM, Leiper JB. Errors in the estimation of hydration status from changes in body mass. J Sports Sci 2007; 25: 797-804
• 26 Miller A, Strauss BJ, Mol S, Kyoong A, Holmes PH, Finlay P, Bardin PG, Guy P. Dual-energy X-ray absorptiometry is the method of choice to assess body composition in COPD. Respirology 2009; 14: 411-418
• 27 Mueller SM, Anliker E, Knechtle P, Knechtle B, Toigo M. Changes in body composition in triathletes during an Ironman race. Eur J Appl Physiol 2013; 113: 2343-2352
• 28 Nana A, Slater GJ, Hopkins WG, Burke LM. Effects of daily activities on dual-energy X-ray absorptiometry measurements of body composition in active people. Med Sci Sports Exerc 2012; 44: 180-189
• 29 Nana A, Slater GJ, Hopkins WG, Burke LM. Effects of exercise sessions on DXA measurements of body composition in active people. Med Sci Sports Exerc 2013; 45: 178-185
• 30 Nana A, Slater GJ, Hopkins WG, Halson SL, Martin DT, West NP, Burke LM. Importance of Standardized DXA Protocol for Assessing Physique Changes in Athletes. Int J Sport Nutr Exerc Metab 2014; [Epub ahead of print]
• 31 Nolte HW, Noakes TD, van VB. Protection of total body water content and absence of hyperthermia despite 2% body mass loss (‘voluntary dehydration’) in soldiers drinking ad libitum during prolonged exercise in cool environmental conditions. Br J Sports Med 2011; 45: 1106-1112
• 32 O’Brien C, Young AJ, Sawka MN. Bioelectrical impedance to estimate changes in hydration status. Int J Sports Med 2002; 23: 361-366
• 33 Olsson KE, Saltin B. Variation in total body water with muscle glycogen changes in man. Acta Physiol Scand 1970; 80: 11-18
• 34 Pastene J, Germain M, Allevard AM, Gharib C, Lacour JR. Water balance during and after marathon running. Eur J Appl Physiol 1996; 73: 49-55
• 35 Refsum HE, Tveit B, Meen HD, Stromme SB. Serum electrolyte, fluid and acid-base balance after prolonged heavy exercise at low environmental temperature. Scand J Clin Lab Invest 1973; 32: 117-122
• 36 Riley WJ, Pyke FS, Roberts AD, England JF. The effect of long-distance running on some biochemical variables. Clin Chim Acta 1975; 65: 83-89
• 37 Sawka MN, Burke LM, Eichner ER, Maughan RJ, Montain SJ, Stachenfeld NS. American College of Sports Medicine position stand. Exercise and fluid replacement. Med Sci Sports Exerc 2007; 39: 377-390
• 38 Sharwood K, Collins M, Goedecke J, Wilson G, Noakes T. Weight changes, sodium levels, and performance in the South African Ironman Triathlon. Clin J Sport Med 2002; 12: 391-399
• 39 Stuempfle KJ, Hoffman MD, Weschler LB, Rogers IR, Hew-Butler T. Race Diet of Finishers and Non-Finishers in a 100 Mile (161 km) Mountain Footrace. J Am Coll Nutr 2011; 30: 529-535
• 40 Tam N, Nolte HW, Noakes TD. Changes in total body water content during running races of 21.1 km and 56 km in athletes drinking ad libitum. Clin J Sport Med 2011; 21: 218-225
• 41 Thomas LW. The chemical composition of adipose tissue of man and mice. Q J Exp Physiol Cogn Med Sci 1962; 47: 179-188
• 42 Thomsen TK, Jensen VJ, Henriksen MG. In vivo measurement of human body composition by dual-energy X-ray absorptiometry (DXA). Eur J Surg 1998; 164: 133-137
• 43 Treuth MS, Butte NF, Wong WW, Ellis KJ. Body composition in prepubertal girls: comparison of six methods. Int J Obes Relat Metab Disord 2001; 25: 1352-1359
• 44 Van Der Ploeg GE, Withers RT, Laforgia J. Percent body fat via DEXA: comparison with a four-compartment model. J Appl Physiol 2003; 94: 499-506
• 45 Van Loan MD. Is dual-energy X-ray absorptiometry ready for prime time in the clinical evaluation of body composition?. Am J Clin Nutr 1998; 68: 1155-1156
• 46 Vieweg WV, Godleski LS, Graham P, Barber J, Kellogg E, Glick J, Hundley PL, Yank GR. The link between fluid intake and weight gain in psychosis. Psychosomatics 1991; 32: 52-57
• 47 Wang Z, Deurenberg P, Wang W, Pietrobelli A, Baumgartner RN, Heymsfield SB. Hydration of fat-free body mass: review and critique of a classic body-composition constant. Am J Clin Nutr 1999; 69: 833-841
• 48 Watts NB. Fundamentals and pitfalls of bone densitometry using dual-energy X-ray absorptiometry (DXA). Osteoporos Int 2004; 15: 847-854
• 49 Yu EW, Thomas BJ, Brown JK, Finkelstein JS. Simulated increases in body fat and errors in bone mineral density measurements by DXA and QCT. J Bone Miner Res 2012; 27: 119-124