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Horm Metab Res 2014; 46(07): 484-489
DOI: 10.1055/s-0034-1370990
Endocrine Research
© Georg Thieme Verlag KG Stuttgart · New York

Aerobic Training Prevents Dexamethasone-Induced Peripheral Insulin Resistance

Authors

  • T. J. Dionísio

    1   Department of Physiological Science, Federal University of São Carlos – UFSCAR, São Carlos, Brazil
    2   Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, USP, Bauru, Brazil

  • J. C. A. Louzada

    3   Department of Physical Education, UNESP – São Paulo State University, Bauru, Brazil

  • B. A. Viscelli

    3   Department of Physical Education, UNESP – São Paulo State University, Bauru, Brazil

  • E. J. Dionísio

    3   Department of Physical Education, UNESP – São Paulo State University, Bauru, Brazil

  • A. M. Martuscelli

    3   Department of Physical Education, UNESP – São Paulo State University, Bauru, Brazil

  • M. Barel

    3   Department of Physical Education, UNESP – São Paulo State University, Bauru, Brazil

  • O. A. B. Perez

    3   Department of Physical Education, UNESP – São Paulo State University, Bauru, Brazil

  • J. R. Bosqueiro

    3   Department of Physical Education, UNESP – São Paulo State University, Bauru, Brazil

  • D. T. Brozoski

    2   Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, USP, Bauru, Brazil

  • C. F. Santos

    2   Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, USP, Bauru, Brazil

  • S. L. Amaral

    1   Department of Physiological Science, Federal University of São Carlos – UFSCAR, São Carlos, Brazil
    3   Department of Physical Education, UNESP – São Paulo State University, Bauru, Brazil
Further Information

Publication History

received 05 November 2013

accepted 19 February 2014

Publication Date:
07 April 2014 (online)

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Abstract

This study investigated how proteins of the insulin signaling cascade could modulate insulin resistance after dexamethasone (Dexa) treatment and aerobic training. Rats were distributed into 4 groups: sedentary control (SC), sedentary+Dexa (SD), trained control (TC), and trained+Dexa (TD), and underwent aerobic training for 70 days or remained sedentary. Dexa was administered during the last 10 days (1 mg · kg−1 per day i. p.). After 70 days, an intraperitoneal glucose tolerance test (ipGTT) was performed. Protein levels of IRS-1, AKT, and PKC-α in the tibialis anterior (TA) muscle were identified using Western blots. Dexa treatment increased blood glucose and the area under the curve (AUC) of ipGTT. Training attenuated the hyperglycemia and the AUC induced by Dexa. Dexa reduced IRS-1 (− 16%) and AKT (− 43%) protein level with no changes in PKC-α levels. Moreover, these effects on IRS-1 and AKT protein level were prevented in trained animals. These results show for the first time that aerobic exercise prevented reductions of IRS-1 and AKT level induced by Dexa in the TA muscle, suggesting that aerobic exercise is a good strategy to prevent Dexa-induced peripheral insulin resistance.

Key words

glucocorticoids - insulin cascade pathway - aerobic exercise training - skeletal muscle