Planta Med 2013; 79(18): 1698-1704
DOI: 10.1055/s-0033-1351098
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Antiallergic Effects of Pigments Isolated from Green Sea Urchin (Strongylocentrotus droebachiensis) Shells

Olga N. Pozharitskaya
1   Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
,
Alexander N. Shikov
1   Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
,
Marina N. Makarova
1   Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
,
Svetlana A. Ivanova
1   Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
,
Vera M. Kosman
1   Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
,
Valery G. Makarov
1   Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
,
Václav Bazgier
2   Department of Physical Chemistry, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic
,
Karel Berka
3   Department of Physical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Olomouc, Czech Republic
,
Michal Otyepka
3   Department of Physical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Olomouc, Czech Republic
,
Jitka Ulrichová
4   Department of Medical Chemistry and Biochemistry, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic
› Author Affiliations
Further Information

Publication History

received 24 June 2013
revised 10 October 2013

accepted 23 October 2013

Publication Date:
28 November 2013 (online)

Abstract

This study was undertaken to evaluate possible antiallergic effects of an extract of pigments from green sea urchin (Strongylocentrotus droebachiensis) shells. Effects were studied on animal models – guinea pig ileum contraction, rabbit eyes allergic conjunctivitis, and rabbit local skin irritation. The extract significantly reduced, in a dose-dependent manner, the histamine-induced contractions of the isolated guinea pig ileum with ID50 = 1.2 µg/mL (in equivalents of spinochrome B), had an inhibitory effect on the model of ocular allergic inflammation surpassing the reference drug olopatadine, and did not show any irritating effect in rabbits. The extract predominantly contained polyhydroxy-1,4-naphthoquinone which would be responsible for the pharmacological activity. The active compounds of the extract were evaluated in silico with molecular docking. Molecular docking into H1R receptor structures obtained from molecular dynamic simulations showed that all spinochrome derivatives bind to the receptor active site, but spinochrome monomers fit better to it. The results of the present study suggest possibilities for the development of new agents for treating allergic diseases on the base of pigments from sea urchins shells.

 
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