Pharmacokinetic Studies of Bergapten in Dog Plasma by Using a LC-MS/MS Method Studies

Abstract

A sensitive LC-MS/MS method was developed for the determination of bergapten in dog plasma. The chromatographic separation was carried out on a Hypersil ODS column with a mobile phase consisting of methanol-water. The plasma sample was precipitated with methanol and prepare for injecting onto the LC-MS/MS system. The detection was performed on a triple quadrupole tandem mass spectrometer by MRM via electro spray ionization source. The standard curve for bergapten was linear over the concentration range of 0.5–500 ng/mL with a lower limit of quantification of 0.5 ng/mL. The inter-day and intra-day precision (R.S.D.%) for bergapten varied between 3.4 and 11.5. The corresponding inter-day and intra-day accuracy (Bias%) ranged between −3.8 and 6.9. For the pharmacokinetic analysis of serum, the mean (SD) values obtained for the bergapten were as follows: C_max, 228.5 (14.3) ng/ml; T_max, 4.2 (0.4) h; t_1/2, 6.9 (2.3) h; AUC_0–t h, 2507.2 (168.5) ng · h/mL and AUC_0–∞, 3 219.2 (211.4) ng · h/mL, respectively.

Key words

bergapten - LC-MS/MS - pharmacokinetics

*

^* These authors contributed equally to this work.

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