Download PDF
Exp Clin Endocrinol Diabetes 2013; 121(05): 306-309
DOI: 10.1055/s-0032-1333298
Article
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Association of Lipid Peroxidation with Endothelial Dysfunction in Patients with Overt Hypothyroidism

B. Tejovathi
1   Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati, Andhra Pradesh, India
,
M. M. Suchitra
1   Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati, Andhra Pradesh, India
,
V. Suresh
2   Endocrinology and Metabolism, Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati, Andhra Pradesh, India
,
V. S. Reddy
1   Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati, Andhra Pradesh, India
,
A. Sachan
2   Endocrinology and Metabolism, Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati, Andhra Pradesh, India
,
P.V.L. N. Srinivas Rao
1   Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati, Andhra Pradesh, India
,
A. R. Bitla
1   Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati, Andhra Pradesh, India
› Author Affiliations
Further Information

Publication History

received 09 August 2012
first decision 19 December 2012

accepted 07 January 2013

Publication Date:
28 February 2013 (online)

Also available at
    Permissions and Reprints

Abstract

Hypothyroidism is associated with increased oxidative stress. The mechanism underlying the endothelial dysfunction in thyroid disease is not yet clear. This study aims to investigate lipid peroxidation and its association with endothelial dysfunction in overt hypothyroidism (OHT).Plasma malondialdehyde (MDA) as a marker of oxidative stress and plasma nitrates and asymmetric dimethyl arginine levels (ADMA) as markers of endothelial dysfunction were estimated in 25 OHT patients in comparison to 25 euthyroid controls. Plasma MDA, ADMA levels were significantly increased, whereas plasma nitrates were significantly decreased in the patient group compared to control group (p<0.01). Moreover, a significant positive association between plasma MDA and ADMA was found in the patient group (ρ=0.472, p=0.036). Our results reveal the presence of endothelial dysfunction in OHT patients as evidenced by decreased plasma nitrates and increased ADMA levels. Increased levels of MDA represent an increased generation of reactive oxygen species in these patients. A finding of significant direct relation of plasma MDA with ADMA indicates that oxidative stress has a strong impact on endothelial dysfunction in overt hypothyroidism. Further studies focusing on the role of oxidative stress in endothelial dysfunction and the effects of antioxidant supplementation on endothelial function in OHT patients are required.

Key words

hypothyroidism - asymmetric dimethyl arginine - malondialdehyde - nitrates - oxidative stress
 

  • References

  • 1 Vallance P, Collier J, Moncada S. Effects of endothelium-derived nitric oxide on peripheral arteriolar tone in man. Lancet 1989; 2: 997-1000
    PubMedGoogle Scholar
  • 2 Vallance P, Leone A, Calver A et al. Accumulation of an endogenous inhibitor of nitric oxide synthesis in chronic renal failure. Lancet 1992; 339: 572-575
    CrossrefPubMedGoogle Scholar
  • 3 Kojda G, Harrison D. Interactions between NO and reactive oxygen species: Pathophysiological importance in atherosclerosis, hypertension, diabetes and heart failure. Cardiovasc Res 1999; 43: 562-571
    CrossrefPubMedGoogle Scholar
  • 4 Cai H, Harrison DG. Endothelial dysfunction in cardiovascular diseases: the role of oxidant stress. Circ Res 2000; 87: 840-844
    CrossrefPubMedGoogle Scholar
  • 5 Napoli R, Biondi B, Guardasole V et al. Impact of hyperthyroidism and its correction on vascular reactivity in humans. Circulation 2001; 104: 3076-3080
    CrossrefPubMedGoogle Scholar
  • 6 Arikan E, Karadag CH, Guldiken S. Asymmetric dimethylarginine levels in thyroid diseases. J Endocrinol Invest 2007; 30: 186-191
    PubMedGoogle Scholar
  • 7 Ozcan O, Cakir E, Yaman H et al. The effects of thyroxine replacement on the levels of serum asymmetric dimethylarginine (ADMA) and other biochemical cardiovascular risk markers in patients with subclinical hypothyroidism. Clin Endocrinol 2005; 63: 203-206
    CrossrefPubMedGoogle Scholar
  • 8 Ojamaa K, Klemperer JD, Klein I. Acute effects of thyroid hormone on vascular smooth muscle. Thyroid 1996; 6: 505-512
    CrossrefPubMedGoogle Scholar
  • 9 Tan FP, Reuters VS, Ferreira MM et al. Lipid profile in different degrees of hypothyroidism and effects of levothyroxine replacement in mild thyroid failure. J Lab Clin Med 2008; 151: 224-231
    PubMedGoogle Scholar
  • 10 Saito I, Saruta T. Hypertension in thyroid disorders. Endocrinol Metab Clin North Am 1994; 23: 379-386
    PubMedGoogle Scholar
  • 11 Nagasaki T, Inaba M, Shirakawa K et al. Increased levels of C-reactive protein in hypothyroid Patients and Its correlation with arterial stiffness in the common carotid artery. Biomed Pharmacother 2007; 61: 167-172
    CrossrefPubMedGoogle Scholar
  • 12 Nedrebø BG, Ericsson UB, Nygård O et al. Plasma total homocysteine ​​levels in hyperthyroid and hypothyroid patients. Metabolism 1998; 47: 89-93
    CrossrefPubMedGoogle Scholar
  • 13 Santi A, Duarte MM, Moresco RN et al. Association between thyroid hormones, lipids and oxidative stress biomarkers in overt hypothyroidism. Clin Chem Lab Med 2010; 48: 1635-1639
    PubMedGoogle Scholar
  • 14 Klein I, Ojamaa K. Thyroid hormone and the cardiovascular system. N Engl J Med 2001; 344: 501-509
    CrossrefPubMedGoogle Scholar
  • 15 Lekakis J, Papamichael C, Alevizaki M et al. Flow-mediated, endothelium-dependent vasodilation is impaired in Subjects with hypothyroidism, borderline hypothyroidism, and high-normal serum thyrotropin (TSH) values. Thyroid 1997; 7: 411-414
    CrossrefPubMedGoogle Scholar
  • 16 Erbil Y, Ozbey N, Giriş M et al. Effects of thyroxine replacement on lipid profile and endothelial function after thyroidectomy. Br J Surg 2007; 94: 1485-1490
    CrossrefPubMedGoogle Scholar
  • 17 Papaioannou G. Treating hypothyroidism Improves endothelial function. Metabolism 2004; 53: 278-279
    CrossrefPubMedGoogle Scholar
  • 18 Shavdatuashvili T. Lipoprotein profile and endothelial function in patients with subclinical and overt hypothyroidism. Georgian Med News 2005; 129: 57-60
    PubMedGoogle Scholar
  • 19 Dagre AG, Lekakis JP, Protogerou AD et al. Abnormal endothelial function in female patients with hypothyroidism and borderline thyroid function. Int J Cardiol 2007; 114: 332-338 Epub 2006 Jun 21
    CrossrefPubMedGoogle Scholar
  • 20 Kocak H, Ceken K, Yavuz A et al. Effect of renal transplantation on endothelial function in haemodialysis patients. Nephrol Dial Transplant 2006; 21: 203-207
    CrossrefPubMedGoogle Scholar
  • 21 Casey DP, Beck DT, Braith RW. Systemic plasma levels of nitrite/nitrate (Nox) reflect brachial flow-mediated dilation responses in young men and women. Clin Exp Pharmacol Physiol 2007; 34: 1291-1293
    CrossrefPubMedGoogle Scholar
  • 22 Engin AB, Sepici-Dincel A, Gonul II et al. Oxidative stress-induced endothelial cell damage in thyroidectomized rat. Exp Toxicol Pathol 2012; 64: 481-485 Epub 2010 Dec 4
    CrossrefPubMedGoogle Scholar
  • 23 Sangeetha P, Das UN, Koratkar R et al. Increase in free radical generation and lipid peroxidation following chemotherapy in patients with cancer. Free radical Bio Med 1990; 8: 15-19
    CrossrefPubMedGoogle Scholar
  • 24 Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by kinetic cadmium reduction method. Clin chem 1990; 36: 1440-1443
    PubMedGoogle Scholar
  • 25 Rajendra W. High performance liquid chromatography determination of amino acids in biological samples by pre column derivatization with O-pthaldialdehyde. J liq chromatogr 1987; 10: 941-955
    PubMedGoogle Scholar
  • 26 Torun AN, Kulaksizoglu S, Kulaksizoglu M et al. Serum total antioxidant status and lipid peroxidation marker malondialdehyde levels in overt and subclinical hypothyroidism. Clin Endocrinol 2009; 70: 469-474
    CrossrefPubMedGoogle Scholar
  • 27 Zoccali C. Asymmetric dimethylarginine in end-stage renal disease patients: A biomarker modifiable by calcium blockade and angiotensin II antagonism?. Kidney Int 2006; 70: 2053-2055
    CrossrefPubMedGoogle Scholar
  • 28 Jun-Hyun Y, Sung-Chang L. Elevated levels of plasma homocyst[e]ine and asymmetric dimethylarginine in elderly patients with stroke. Atherosclerosis 2001; 158: 425-430
    CrossrefPubMedGoogle Scholar
  • 29 Cardounel AJ, Cui H, Samouilov A et al. Evidence for the pathophysiological role of endogenous methylarginines in regulation of endothelial NO production and vascular function. J Biol Chem 2007; 282: 879-887
    CrossrefPubMedGoogle Scholar
  • 30 Higashi Y, Noma K, Yoshizumi M et al. Endothelial Function and Oxidative Stress in Cardiovascular Diseases. Circ J 2009; 73: 411-418
    CrossrefPubMedGoogle Scholar
  • 31 Stehouwer CDA, Lambert J, Donker AJM et al. Endothelial dysfunction and pathogenesis of diabetic angiopathy. Cardiovas Res 1997; 34: 55-68
    CrossrefPubMedGoogle Scholar
  • 32 Clausen P, Mersebach H, Nielsen B et al. Hypothyroidism is associated with signs of endothelial dysfunction despite 1-year replacement therapy with levothyroxine. Clin Endocrinol (Oxf) 2009; 70: 932-937 Epub 2008 Sep 5
    CrossrefPubMedGoogle Scholar
  • 33 Lane HA, Smith JC, Davies JS. Noninvasive assessment of preclinical atherosclerosis. Vasc Health Risk Manag 2006; 2: 19-30
    CrossrefPubMedGoogle Scholar