Abstract
Four (1–4) previously unknown lupane-type triterpenoidal
saponins were isolated from the roots of Pulsatilla chinensis, along
with six triterpene saponins (5–10). The structures of
saponins 1–4 were determined as
23-hydroxy-3β-[(O-β-D-glucopyranosyl-(1 → 4)-α-L-arabinopyranosyl)]lup-20(29)-en-28-oic-acid
28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester
(1),
23-hydroxy-3β-[(O-α-L-rhamnopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1 → 4)]-α-L-arabinopyranosyl)]lup-20(29)-en-28-oic-acid
28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester
(2),
3β-[(O-β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl)]lup-20(29)-en-28-oic-acid
28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester
(3), and
3β-[(O-β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl)]lup-20(29)-en-28-oic-acid
28-O-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl
ester (4), on the basis of hydrolysis and spectral evidence,
including 1D- and 2D-NMR and HR-ESI-MS analyses. These pure isolates
(1–10) were tested for their anticomplement activity,
using an in vitro assay of the complement system of the classical
pathway.
Key words
Pulsatilla chinensis
- Ranunculaceae - triterpenoidal saponins - lupine - anti-complementary activity -
classical pathway
