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Planta Med 2012; 78(16): 1757-1760
DOI: 10.1055/s-0032-1315257
Letters
Georg Thieme Verlag KG Stuttgart · New York

The In Vitro Protective Effects of Curcumin and Demethoxycurcumin in Curcuma longa Extract on Advanced Glycation End Products-Induced Mesangial Cell Apoptosis and Oxidative Stress

Authors

  • Ji-ping Liu

    1   Department of Pharmacology, Shaanxi University of Chinese Medicine, Xianyang, P. R. China

  • Liang Feng

    2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China

  • Mao-mao Zhu

    2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China

  • Ru-Shang Wang

    2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China

  • Ming-hua Zhang

    2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China

  • Shao-ying Hu

    2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China

  • Xiao-bin Jia

    2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China

  • Jin-Jie Wu

    3   Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Jiangsu Nanjing, P. R. China
Further Information

Publication History

received 26 February 2012
revised 24 July 2012

accepted 26 July 2012

Publication Date:
24 August 2012 (online)

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Abstract

Curcuma longa L. (CLL), a traditional herbal medicine, has been widely used for the prevention of diabetic vascular complications in recent years. However, the protective effects of curcuminoids in CLL on the AGEs-induced damage to mesangial cell are not fully understood. In this present study, dihydroethidium, superoxide dismutase kit, malondialdehyde kit, and acridine orange/ethidium bromide staining methods were used to evaluate the activities of curcumin and demethoxycurcumin (10−11 − 10−9 M) on AGEs-induced oxidative stress and apoptosis, which were associated with the damage to mesangial cell. The results showed that these two compounds could significantly restore advanced glycation end products (AGEs)-induced apoptosis to normal levels (IC50 = 3.874 × 10−11 M for curcumin and IC50 = 6.085 × 10−11 M for demethoxycurcumin) and reduce remarkably reactive oxygen species generation in mesangial cell. Furthermore, curcumin and demethoxycurcumin dramatically elevated AGEs-decreased superoxide dismutase activity while significantly reducing AGEs-increased malondialdehyde content in cell culture supernatant. Our results suggest that both curcumin and demethoxycurcumin have a significant protective potential to the prevention of diabetic nephropathy.

Key words

Curcuma longa L. - Zingiberaceae - curcumin - demethoxycurcumin - diabetic nephropathy - anti-apoptosis - antioxidant

Supporting Information

 

  • References

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