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Arzneimittelforschung 2008; 58(7): 336-341
DOI: 10.1055/s-0031-1296516
Liver Therapeutics
Editio Cantor Verlag Aulendorf (Germany)

Simultaneous Determination of 6,7-Dimethylesculetin and Geniposide in Rat Plasma and its Application to Pharmacokinetic Studies of Yin Chen Hao Tang Preparation

Xijun Wang
1   Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Haitao Lv
1   Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Hui Sun
1   Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Wenjun Sun
1   Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Lian Liu
1   Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Ping Wang
1   Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Hongxin Cao
2   China Academy of Chinese Medical Science, Beijing, P. R. China
› Author Affiliations
Further Information

Publication History

Publication Date:
15 December 2011 (online)

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Abstract

A method for the rapid and simultaneous determination of 6,7-dimethylesculetin (CAS 120-08-1) and geniposide (CAS 24512-63-8) in rat plasma has been developed, using validated high performance liquid chromatography (HPLC) with solid phase extraction (SPE). The HPLC analysis was performed on a commercially available column (200 mm × 4.6 mm, 5 µm) with acetonitrile-methanol −0.1% aqueous formic acid as mobile phase and the UV detection at 343 nm and 238 nm for 6,7-dimethylesculetin and geniposide, respectively. The calibration curves for 6,7-dimethylesculetin and geniposide were linear over the range 0.4–25.6 µg/mL and 1.12–71.68 µg/mL, respectively. The lower limits of quantitation were 0.40 µg/mL and 1.12 µg/mL, and the lower limits of detection were 0.06 µg/mL and 0.09 µg/mL, respectively. The intra-day and inter-day precision for 6,7-dimethylesculetin and geniposide were < 5%, whereas the absolute recovery percentages were > 74%. A successful application of the developed HPLC analysis was demonstrated for the pharmacokinetic study of a Traditional Chinese Medicine formula of Yin Chen Hao Tang preparation.

Key words

CAS 120-08-1 - CAS 24512-63-8 - 6,7-Dimethylesculetin, high performance liquid chromatography, pharmacokinetics, rat - Geniposide, high performance liquid chromatography, pharmacokinetics, rat - Yin Chen Hao Tang preparation, pharmacokinetics
 

  • References

  • 1 Li Q, Jia Y, Sun LX, Xu L, Tong L, Shen ZD et al. High-Performance Liquid Chromatographic Method for Isofraxidin in Rat Plasma. Chromatographia. 2006; 3: 249-253
    PubMedGoogle Scholar
  • 2 Feng SL, Hu FD, Zhao JX, Liu X, Li Y. Determination of eleutheroside E and eleutheroside B in rat plasma and tissue by high-performance liquid chromatography using solid-phase extraction and photodiode array detection. J Pharm Biopharm. 2006; 62: 315-320
    CrossrefPubMedGoogle Scholar
  • 3 Wang XJ, Sun H, Fan YL, Li LJ, Makino T, Kano Y. Analysis and bioactive evaluation of the compounds absorbed into blood after oral administration of the extracts of Vaccinium vitis-idaea in rat. Biol Pharm Bull. 2005; 28: 1106-1108
    CrossrefPubMedGoogle Scholar
  • 4 Zhao YL, Wang XY, Zhao YC, Gao XX, Bi KS, Yu ZG. HPLC Determination and Pharmacokinetic Study of Homoerio-dictyol-7-O-ß-D-glucopyranoside in Rat Plasma and Tissues. Biol Pharm Bull. 2007; 30: 617-620
    CrossrefPubMedGoogle Scholar
  • 5 Shao F, Wang GJ, Xie HT, Zhu XY, Sun JG, Jiye A. Pharmacokinetic Study of Triptolide, a Constituent of Immunosuppressive Chinese Herb Medicine, in Rats. Biol Pharm Bull. 2007; 30: 702-707
    CrossrefPubMedGoogle Scholar
  • 6 Makino T, Inagaki T, Komatsu K, Kano Y. Pharmacokinetic interactions between Japanese traditional kampo medicine and modern medicine (IV)-Effect of Kamisyoyosan and Tokisyakuyakusan on the pharmacokinetics of etizolam in rats. Biol Pharm Bull. 2005; 28: 280-284
    CrossrefPubMedGoogle Scholar
  • 7 Yoshioka M, Ohnishi N, Sone N, Egami S, Takara K, Yokoyama T et al. Studies on Interactions between Functional Foods or Dietary Supplements and Medicines. III. Effects of Ginkgo biloba Leaf Extract on the Pharmacokinetics of Nifedipine in Rats. Biol Pharm Bull. 2004; 27: 2042-2045
    CrossrefPubMedGoogle Scholar
  • 8 Arai M, Yokosuka O, Fukai K, Kanda T, Kojima H, Kawai S et al. A case of severe acute hepatitis of unknown etiology treated with the Chinese herbal medicine Inchinko-to. Hepatol Res. 2004; 28: 161-165
    CrossrefPubMedGoogle Scholar
  • 9 Imanishi Y, Maeda N, Otogawa K, Seki S, Matsui H, Kawada N et al. Herb medicine Inchin-ko-to (TJ-135) regulates PDGF-BB-dependent signaling pathways of hepatic stellate cells in primary culture and attenuates development of liver fibrosis induced by thioacetamide administration in rats. J. Hepatol. 2004; 41: 242-250
    CrossrefPubMedGoogle Scholar
  • 10 Inao M, Mochida S, Matsui A, Eguchi Y, Yulutuz Y, Wang YH et al. Japanese herbal medicine Inchin-ko-to as a therapeutic drug for liver fibrosis. J. Hepatol. 2004; 41: 584-591
    CrossrefPubMedGoogle Scholar
  • 11 Sakaida I, Tsuchiya M, Kawaguchi K, Kimura T, Terai S, Okita K. Herbal medicine Inchin-ko-to (TJ-135) prevents liver fibrosis and enzyme altered lesions in rat liver cirrhosis induced by a choline-deficient Lamino acid-defined diet. J. Hepatol. 2003; 38: 762-769
    CrossrefPubMedGoogle Scholar
  • 12 Chang YC, Chou FP Huang HP, Hsu JD, Wang CJ. Inhibition of cell cycle progression by penta-acetyl geniposide in rat C6 glioma cells. Toxicol Appl Pharmacol. 2004; 198: 11-20
    CrossrefPubMedGoogle Scholar
  • 13 Zhang LM, He KZ, Ren ZJ, Pu Q. Biochemical mechanism of hepaticprotective effect of Geniposide on liver injured mice induced by CCL4. Chin J Appl Environ Biol. 2005; 11: 669-672
    PubMedGoogle Scholar
  • 14 Kano Y, Wang XJ, Junko S, Reiko M. Pharmacological properties of galenical prepration (XIX) Pharmacokinetics study of 6,7-dimethylesculetin in rats. J Trad Med. 1994; 11: 176-180
    PubMedGoogle Scholar
  • 15 Wang XJ, Li TL, Sun H. Hepatic protection of 6,7-dimethylesculetin: constituents absorbed into blood of Yinchenha-otang and Yinchenhaotang. Chin Pharmacol Bull. 2004; 20: 239-240
    PubMedGoogle Scholar
  • 16 Wang XJ, Sun H, Hua YS, Komatsu K. Studies on the distribution of 3H-Dimethylesculetin and its excretion in rats. J Natural Medicines. 1999; 63: 101-104
    PubMedGoogle Scholar
  • 17 Wang Q, Yu ZG, Xu S. Research on the pharmacokinetic parameters of 6,7-dimethoxycoumarin after oral administration of Yinchenhao and its decoction. Zhongguo Zhong Cheng Yao. 2003; 25: 182-185
    PubMedGoogle Scholar
  • 18 Yu ZG, Bi KS, Wang Q. The study on main metabolites of 6,7-dimethylesculetin in rats body. World Sci Technol Mod Trad Chin Med. 2003; 5: 40-45
    PubMedGoogle Scholar
  • 19 Xu L, Zhang L, Li Q, Li XQ, Chen XH, Bi KS. Determination of Geniposide in Rats Plasma. Application to Pharmacokinetic Studies of Sendeng-4 Decoction. Chromatographia. 2006; 63: 9-10
    PubMedGoogle Scholar
  • 20 Ye G, Zhu HY, Zhao HL, Xu B, Huang CG. HPLC method for the determination and pharmacokinetic studies on geniposide in rat serum after oral administration of traditional Chinese medicinal preparation Yin-Zhi-Ku decoction. Biomed Chromatogr. 2005; 20: 743-747
    PubMedGoogle Scholar
  • 21 Tseng TY, Tsai TH. Measurement of unbound geniposide in blood, liver, brain and bile of anesthetized rats: an application of pharmacokinetic study and its influence by the acupuncture. Anal Chem Acta. 2004; 517: 47-52
    CrossrefPubMedGoogle Scholar
  • 22 Xia SX, Zhang YL, Zhang H, Bao YL, Xiang SJ, Li XH. Determination of geniposide in rat plasma and its pharmacokinetics. Zhongguo Zhong Yi Yao Xue Kan. 2004; 22: 1088-1089
    PubMedGoogle Scholar
  • 23 Zhang ZJ. Shanghanlun. Beijing: Renmin Weisheng Publisher; 2005: 8
    Google Scholar
  • 24 Han GZ. Pharmacokinetics of Chinese traditional and herbal drugs. Beijing: China Medicine and Science Publisher; 1999
    Google Scholar