Quantification of quetiapine in human plasma by reverse phase high performance liquid chromatography

Abstract

The objective of the present investigation is to develop and validate a simple, economical and reliable high performance liquid chromatography method for the quantification of quetiapine (CAS 111974-72-2) in human plasma with a quantification limit sufficiently low to support pharmacokinetic studies. Imipramine hydrochloride (CAS 113-52-0) was used as internal standard. The validated method allows quantification of quetiapine in 15–1000 ng/mL. The method was shown to be precise (≤ 7% coefficient of variation, CV) and accurate (≤ 10% relative error, RE). The correlation coefficient for quetiapine was > 0.9970. The simplicity of the assay and rapid liquid-liquid extraction make it an attractive procedure in high-throughput bioanalysis of quetiapine.

Key words

Antipsychotics - CAS 113-52-0 - CAS 111974-72-2 Imipramine - Quetiapine, bioanalysis - Reverse phase HPLC with UV detection

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