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Int J Angiol 2011; 20(3): 157-166
DOI: 10.1055/s-0031-1284475
ORIGINAL ARTICLE

© Thieme Medical Publishers

Cyclic Strain Delays the Expression of Tissue Factor Induced by Thrombin in Human Umbilical Vein Endothelial Cells

Norio Yamashita1 , Ryuzo Abe1 , Alexander M. Nixon1 , Adrienne L. Rochier1 , Joseph A. Madri2 , Bauer E. Sumpio1
  • 1Department of Surgery, Yale University School of Medicine and Connecticut Veterans Administration Health Care System, New Haven, CT
  • 2Department of Pathology, Yale University School of Medicine, New Haven, CT
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Publikationsverlauf

Publikationsdatum:
19. Juli 2011 (online)

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ABSTRACT

Most studies of tissue factor (TF) expression in endothelial cells (EC) are performed under stationary culture conditions. The purpose of this study was to determine the influence of mechanical stimuli such as cyclic strain (CS) on the expression of TF in EC exposed to thrombin (Thr). Human umbilical vein endothelial cells (HUVEC) were exposed to 4 U·mL−1 Thr in the presence or absence of 10% average CS at 60 cycles·min−1 and then TF expression was measured. TF messenger RNA (mRNA) expression peaked at 2 hours in HUVEC exposed to Thr, but at 4 hours in HUVEC exposed to both Thr + CS. TF expression was inhibited by p38 and extracellular signal-regulated protein kinase (ERK) inhibitors. For both Thr or Thr + CS stimuli, p38 and ERK activity peaked at 5 minutes (p < 0.05). Nuclear factor-kappa B levels remained high in the Thr group but not in the Thr + CS group, while Egr-1 levels were elevated in the Thr + CS group. We demonstrated CS-delayed, Thr-induced TF mRNA expression in HUVEC, which may be modulated by p38 and ERK inhibitors.

KEYWORDS

Hemodynamic forces - thrombin - endothelium - tissue factor - atherosclerosis

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