Abstract
High-throughput natural product research produced a suite of anticancer hits among
several species of the Orchidaceae family (Oncidium microchilum, O. isthmi , and Myrmecophila humboldtii ). A commercial Oncidium sp. was also examined as a convenient source of additional material. Isolation and
structure elucidation led to the identification of fifteen stilbenoids including a
new phenanthraquinone and two new dihydrostilbenes. NMR data for structure elucidation
and dereplication were acquired utilizing a Bruker BioSpin TCI 1.7-mm MicroCryoProbe
or a 5-µL CapNMR capillary microcoil. Several compounds inhibited proliferation of
NCI-H460 and M14 cancer cell lines. All compounds were also examined for their ability
to induce apoptosis. Apoptosis induction was determined by measuring caspase 3/7 activation
and LDH release in a NCI-H460 cell line. Based on these results, a portion of the
extract from a commercially available Oncidium sp. was chemically modified in an attempt to obtain additional phenanthraquinones.
Key words
Orchidaceae -
Oncidium
- Myrmecophila - phenanthraquinone - stilbenoid - apoptosis
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Dr. Russell B. Williams
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USA
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Email: rwilliams@sequoiasciences.com