Planta Med 2011; 77(18): 2057-2060
DOI: 10.1055/s-0031-1280097
Natural Product Chemistry
Letters
© Georg Thieme Verlag KG Stuttgart · New York

Cytotoxic Angucyclines from Amycolatopsis sp. HCa1, a Rare Actinobacteria Derived from Oxya chinensis

Zhi Kai Guo1 , Ting Wang1 , Ye Guo1 , Yong Chun Song1 , Ren Xiang Tan1 , Hui Ming Ge1 , 2
  • 1Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China
  • 2State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai, China
Further Information

Publication History

received February 20, 2011 revised June 18, 2011

accepted June 28, 2011

Publication Date:
09 August 2011 (online)

Abstract

Two new angucyclines, named (2R,3R)-2-hydroxy-8-O-methyltetrangomycin (1) and (2R,3R)-2-hydroxy-5-O-methyltetrangomycin (2), together with eight known compounds (310), were isolated from the culture of Amycolatopsis sp. HCa1, a rare actinobacteria isolated from the gut of Oxya chinensis. The new structures were elucidated through extensive spectroscopic data analysis, and their absolute configurations were assigned by application of the modified Mosher's method and CD spectrum comparison. Their in vitro cytotoxic activities against four cell lines including human cervical cancer cell line (HeLa), human gastric adenocarcinoma cell line (SGC-7901), human lung adenocarcinoma cell line (SPC-A-1), and mouse macrophage cell line (RAW264.7) were then investigated. Compounds 3, 4, 9, and 10 showed potent cytotoxic activities towards the HeLa cells with IC50 values of 0.27, 0.11, 0.56, and 0.39 µM, respectively.

Supporting Information

References

  • 1 Oh D C, Poulsen M, Currie C R, Clardy J. Dentigerumycin: a bacterial mediator of an ant-fungus symbiosis.  Nat Chem Biol. 2009;  5 391-393
  • 2 Scott J J, Oh D C, Yuceer M C, Klepzig K D, Clardy J, Currie C R. Bacterial protection of beetle-fungus mutualism.  Science. 2008;  322 63
  • 3 Currie C R, Scott J A, Summerbell R C, Malloch D. Fungus-growing ants use antibiotic-producing bacteria to control garden parasites.  Nature. 1999;  398 701-704
  • 4 Iwai K, Iwamoto S, Aisaka K, Suzuki M. Isolation of novel actinomycetes from spider materials.  Actinomycetologica. 2009;  23 8-15
  • 5 Zhang Y L, Ge H M, Zhao W, Dong H, Xu Q, Li S H, Li J, Zhang J, Song Y C, Tan R X. Unprecedented immunosuppressive polyketides from Daldinia eschscholzii, a mantis-associated fungus.  Angew Chem Int Ed. 2008;  47 5823-5826
  • 6 Kunimoto S, Lu J, Esumi H, Yamazaki Y, Kinoshita N, Honma Y, Hamada M, Ohsono M, Ishizuka M, Takeuchi T. Kigamicins, novel antitumor antibiotics. I. Taxonomy, isolation, physico-chemical properties and biological activities.  J Antibiot. 2003;  56 1004-1011
  • 7 Matsumoto N, Tsuchida T, Sawa R, Iinuma H, Nakamura H, Naganawa H, Sawa T, Takeuchi T. Epoxyquinomicins A, B, C and D, new antibiotics from Amycolatopsis. III. Physico-chemical properties and structure determination.  J Antibiot. 1997;  50 912-915
  • 8 Tsuchida T, Sawa R, Takahashi Y, Iinuma H, Sawa T, Naganawa H, Takeuchi T. Azicemicins A and B, new antimicrobial agents produced by Amycolatopsis. II. Structure determination.  J Antibiot. 1995;  48 1148-1152
  • 9 Zhu L L, Ostash B, Rix U, Nur-e-Alam M, Mayers A, Luzhetskyy A, Mendez C, Salas J A, Bechthold A, Fedorenko V, Rohr J. Identification of the function of gene lndM2 encoding a bifunctional oxygenase-reductase involved in the biosynthesis of the antitumor antibiotic landomycin E by Streptomyces globisporus 1912 supports the originally assigned structure for landomycinone.  J Org Chem. 2005;  70 631-638
  • 10 Kuntsmann M P, Mitscher L A. The structural characterization of tetrangomycin and tetrangulol.  J Org Chem. 1966;  31 2920-2925
  • 11 Kern D L, Schaumberg J P, Hokanson G C, French J C. PD 116 779, a new antitumor antibiotic of the benz[α]anthraquinone class.  J Antibiot. 1986;  39 469-470
  • 12 Yamashita N, Harada T, Shin-Ya K, Seto H. 6-Hydroxytetrangulol, a new CPP32 protease inducer produced by Streptomcyces sp.  J Antibiot. 1998;  51 79-81
  • 13 Maehr H, Liu C M, Liu M, Perrotta A, Smallheer J M, Williams T H, Blount J F. Microbial products. VI. Five novel metabolites related to benz[α]anthracene from an unidentified actinomycete designated X-14881.  J Antibiot. 1982;  35 1627-1631
  • 14 Gould S J, Cheng X C. New benz[α]anthraquinone secondary metabolites from Streptomyces phaeochromogenes.  J Org Chem. 1994;  59 400-405
  • 15 Irie H, Mizuno Y, Kouno I, Nagasawa T, Tani Y, Yamada H, Taga T, Osaki K. Structures of new antibiotic substances, Sakyomicin A, B, C and D; X-ray crystal and molecular structure of Sakyomicin A.  J Chem Soc Chem Commun. 1983;  174-175
  • 16 Li E W, Tian R R, Liu S C, Chen X L, Guo L D, Che Y S. Pestalotheols A–D, bioactive metabolites from the plant endophytic fungus Pestalotiopsis theae.  J Nat Prod. 2008;  71 664-668
  • 17 Rohr J, Thiericke R. Angucycline group antibiotics.  Nat Prod Rep. 1992;  9 103-137
  • 18 Shigihara Y, Koizumi Y, Tamamura T, Homma Y, Isshiki K, Dobashi K, Naganawa H, Takeuchi T. 6-Deoxy-8-O-methylrabelomycin and 8-O-methylrabelomycin from a Streptomyces species.  J Antibiot. 1988;  41 1260-1264
  • 19 Gilpin M L, Balchin J, Box S J, Tyler J W. MM 47755, a new benz[α]anthracene antibiotic from a Streptomycete.  J Antibiot. 1989;  42 627-628
  • 20 Kesenheimer C, Groth U. Total synthesis of (-)-8-O-methyltetrangomycin (MM 47755).  Org Lett. 2006;  8 2507-2510
  • 21 Ge H M, Yu Z G, Zhang J, Wu J H, Tan R X. Bioactive alkaloids from endophytic Aspergillus fumigatus.  J Nat Prod. 2009;  72 753-755

Assoc. Prof. Dr. Hui Ming Ge

Institute of Functional Biomolecules
State Key Laboratory of Pharmaceutical Biotechnology
Nanjing University

22 Hankou Road

Nanjing 210093

China

Phone: +86 25 83 59 32 01

Fax: +86 25 83 59 32 01

Email: hmge@nju.edu.cn