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Horm Metab Res 2011; 43(4): 261-267
DOI: 10.1055/s-0030-1270452
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Changes of CCAAT Enhancer-binding Proteins (CEBPs) in the Lung of Streptozotocin-induced Diabetic Rats

Y. Fong1 , [*] , K.-H. Shen2 , 3 , [*] , L.-J. Chen4 , J.-T. Cheng3 , 4
  • 1Department of Surgery, Chi-Mei Medical Center, Yong Kang City, Tainan County, Taiwan, R. O. C.
  • 2Department of Urology, Chi-Mei Medical Center, Yong Kang City, Tainan County, Taiwan, R. O. C.
  • 3Department of Medical Research, Chi-Mei Medical Center, Yong Kang City, Tainan County, Taiwan, R. O. C.
  • 4Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan City, Taiwan, R. O. C.
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Publication History

received 28.10.2010

accepted 02.12.2010

Publication Date:
19 January 2011 (online)

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Abstract

CCAAT enhancer-binding proteins (CEBPs) play key roles in the metabolic regulation, cell transformation, and inflammation. However, the expression and/or functions of CEBPs in rats with hyperglycemia are still unclear. In the present study, we investigated the changes of CEBPs protein in lung of the diabetic rats. The levels of C/EBPβ and C/EBPδ protein were decreased in the lung isolated from streptozotocin-induced diabetic rats (STZ-diabetic rats) as compared with that of normal rats. Exogenous insulin at the dose sufficient to normalize the plasma glucose of STZ-diabetic rats reversed the protein levels of C/EBPβ and C/EBPδ in lung after a 4-day treatment. Similar results were also observed in STZ-diabetic rats that received the treatment of phlorizin to reverse the plasma glucose level for 4 days. Otherwise, the protein level of C/EBPα in lung of the STZ-diabetic rats was similar as the normal rats. Also, the level of C/EBPα protein in lung of the STZ-diabetic rat was not significantly changed by correction of plasma glucose by exogenous insulin or phlorizin. In addition, we also cultured human lung cells (A-549) and rat lung cells (L2) in varies concentration of D-glucose and L-glucose to identify the effect of glucose in expression of C/EBPs. The obtained results suggest that increase of plasma glucose is related to the lower expression of C/EBPβ and C/EBPδ proteins in the lung of STZ-diabetic rats. The changes of expression of C/EBPβ and C/EBPδ are not caused by changes of osmolarity but by D-glucose itself.

Key words

lung - CCAAT enhancer-binding proteins (CEBPs) - phlorizin - STZ-diabetic rats - D-glucose - L-glucose

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1 These authors contributed equally to this study.

Correspondence

Prof. J.-T. ChengPhD, FCP 

Department of Medical

Research

Chi-Mei Medical Center

Yong Kang City

Tainan County

73101 Taiwan

R. O. C.

Phone: +886/6/237 2706

Fax: +886/6/238 6548

Email: m980103@mail.chimei.org.tw