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Planta Med 2010; 76(16): 1827-1833
DOI: 10.1055/s-0030-1250039
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Andrographolide Exhibits Anti-Invasive Activity against Colon Cancer Cells via Inhibition of MMP2 Activity

Hsueh-Ping Chao1 , Cheng-Deng Kuo2 , Jen-Hwey Chiu1 , Shu-Ling Fu1
  • 1Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan
  • 2Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan
Further Information

Publication History

received Dec. 15, 2009 revised February 10, 2010

accepted May 11, 2010

Publication Date:
10 June 2010 (online)

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Abstract

Andrographolide, a major constituent of Andrographis paniculata, was previously shown to exhibit anti-inflammatory, antiviral, and anticancer activities. The anticancer activity of andrographolide includes growth suppression, apoptosis promotion, antiangiogenesis, and antitransformation. However, the effect of andrographolide on cancer metastasis, the most malignant feature of cancer, has not been elucidated extensively. In the present study, we demonstrated that andrographolide at nontoxic to subtoxic concentrations (0.3–3 µM) suppressed the invasion ability of CT26 cells in Matrigel-based invasion assays. In addition, the expression of cell adhesion regulators (β-catenin and ILK) was not altered by andrographolide treatment. However, andrographolide indeed inhibited matrix metalloproteinase 2 (MMP2) activity without affecting its expression. Furthermore, the activation of ERK, but not Akt, was attenuated by andrographolide treatment. Notably, a similar inhibitory effect of andrographolide on the invasion and MMP2 activity of the human colon cancer cell line HT29 was also observed. In summary, our results indicate that andrographolide exhibits anti-invasive activity against colon cancer cells via inhibition of MMP2 activity.

Key words

andrographolide - Andrographis paniculata - Acanthaceae - anticancer - invasion - MMP2

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Shu-Ling Fu

Institute of Traditional Medicine
National Yang-Ming University

155, Sec.2, Li-Nong St.

Taipei 11221

Taiwan

Phone: + 88 62 28 26 71 77

Fax: + 88 62 28 22 50 44

Email: slfu@ym.edu.tw

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