Abstract
Phytochemical investigations of Cotinus coggygria Scop. wood, a medicinal and tinctorial plant used since antiquity, resulted in the
isolation and structure elucidation of the novel C-3/C-3′′ dimer of butin (3′,4′,7-trihydroxyflavanone)
and other known compounds: gallic acid and its methyl ester; catechin; profisetinidins:
fisetinidol-(4α →8)-(+)-catechin and epifisetinidol-(4β →8)-(+)-catechin; flavanonols: fustin and dihydroquercetagetin; flavanones: butin
and eriodictyol; flavonols: fisetin and quercetin; the chalcone butein and the aurone
sulfuretin. The isolated compounds were used for the development and validation of
a HPLC-method which enables the determination of these bioactive substances in C. coggygria extracts. Separation was possible on an ether-linked phenyl column material, using
as mobile phase mixtures of water, methanol, and acetonitrile with 0.02 % trifluoroacetic
acid. Sensitivity, selectivity, linearity, precision, accuracy, and repeatability
of the method were verified and assured suitability for its intended use. LC‐MS experiments
performed in positive and negative electrospray ionization mode confirmed the identity
of analytes and allowed unambiguous assignment of all peaks of interest. The analysis
of different C. coggygria samples revealed that sulfuretin (0.38–0.69 %) and fustin (0.33–0.59 %) dominated,
followed by dihydroquercetagetin (0.12–0.35 %), a rare flavanonol derivative with
a 5,6,7-trihydroxysubstituted A-ring. The new natural compound C-3/C-3′′ flavanone
dimer occurred in concentrations of 0.03–0.06 %; the two latter compounds could represent
valuable markers for the identification and quality control of C. coggygria wood.
Key words
Cotinus coggygria
- Anacardiaceae - HPLC‐DAD - LC‐MS - validation - biflavonoids
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diana.antal@umft.ro
