Abstract
A new cytotoxic roridin-type trichothecene macrolide named roritoxin E (1) was characterized from the solid culture of Myrothecium roridum IFB-E091 (residing originally inside Artemisia annua root), together with the known compounds lumichrome (2), (22E,24S)-cerevisterol (3), and (22E,24R)-6β-methoxy-ergosta-7,22-diene-3β,5α-diol (4). The structure of the new macrolide (1) was elucidated by a combination of spectroscopic (UV, IR, MS, and 1D and 2D NMR)
analyses. Compound 1 was demonstrated to be inhibitory in vitro against the gastric carcinoma SGC-7901 and hepatocarcinoma SMMC-7721 cell lines,
with IC50 values of 0.26 and 10.54 µg/mL, respectively. 5-Fluorouracil co-assayed as a positive
control had an IC50 value of 6.66 µg/mL against SGC-7901 cells, and it demonstrated only a 9.98 % growth
inhibition against SMMC-7721 cells at 10 µg/mL.
Key words
Myrothecium roridum
- Tuberculariaceae - endophyte - trichothecene macrolide - roritoxin E
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Prof. Ren Xiang Tan
Institute of Functional Biomolecules
State Key Laboratory of Pharmaceutical Biotechnology
Nanjing University
Nanjing 210093
People's Republic of China
Phone: + 86 25 83 59 32 01
Fax: + 86 25 83 59 32 01
Email: rxtan@nju.edu.cn
