A Neutral Glucan Extracted from Dried Ginger (Zingiberis Rhizoma): Preparation, Structure Characterization, and Immunomodulatory Activity

Abstract

A neutral glucan, GJ0D, was obtained from dried ginger (Zingiberis rhizoma) by enzymatic extraction and purification with column chromatography. The fine structure of GJ0D was assessed through monosaccharide composition analysis, methylation, and two-dimensional nuclear magnetic resonance. GJ0D has a relative molecular weight of 4.0 KDa and possesses a backbone consisting of 1,4-linked α-Glcp with substitution at C-6 of Glcp by T-Glcp. Immunoactivity assessment showed that GJ0D significantly upregulates the expression of IL-6, IL-1β, and TNF-α in RAW264.7 cells. The reactive oxygen species (ROS) production was also increased in RAW264.7 cells. In addition, the expression of several proteins associated with immune activation signaling pathways including TLR4, the phosphorylation of IKKβ, and NF-κB (p100 and p52) were significantly upregulated by GJ0D. These results suggest that GJ0D could promote inflammation through the TLR4/IKKβ/P100 signaling pathway, suggesting a potential application as an immunomodulating agent.

Keywords

ginger - Zingiber officinale - Zingiberaceae - structure characterization - immunomodulatory activity - glucan

Publication History

Received: 11 November 2024

Accepted: 20 March 2025

Article published online:
14 April 2025

• References

• 1 Srinivasan K. Ginger rhizomes (Zingiber officinale): A spice with multiple health beneficial potentials. PharmaNutrition 2017; 5: 18-28
• 2 Gupta S, Sharma A. Medicinal properties of Zingiber officinale Roscoe – A Review. IOSR-JPBS 2014; 9: 124-129
• 3 Mao QQ, Xu XY, Cao SY, Gan RY, Corke H, Beta T, Li HB. Bioactive compounds and bioactivities of ginger (Zingiber officinale Roscoe). Foods 2019; 8: 185
• 4 Ballester P, Cerdá B, Arcusa R, Marhuenda J, Yamedjeu K, Zafrilla P. Effect of ginger on inflammatory diseases. Molecules 2022; 27: 7223
• 5 Liao DW, Cheng C, Liu JP, Zhao LY, Huang DC, Chen GT. Characterization and antitumor activities of polysaccharides obtained from ginger (Zingiber officinale) by different extraction methods. Int J Biol Macromol 2020; 152: 894-903
• 6 Yang X, Wei S, Lu X, Qiao X, Simal-Gandara J, Capanoglu E, Woźniak Ł, Zou L, Cao H, Xiao J, Tang X, Li N. A neutral polysaccharide with a triple helix structure from ginger: Characterization and immunomodulatory activity. Food Chem 2021; 350: 129261
• 7 Chen X, Wang Z, Kan J. Polysaccharides from ginger stems and leaves: Effects of dual and triple frequency ultrasound assisted extraction on structural characteristics and biological activities. Food Biosci 2021; 42: 101166
• 8 Chen X, Chen G, Wang Z, Kan J. A comparison of a polysaccharide extracted from ginger (Zingiber officinale) stems and leaves using different methods: preparation, structure characteristics, and biological activities. Int J Biol Macromol 2020; 151: 635-649
• 9 Nonaka K, Bando M, Sakamoto E, Inagaki Y, Naruishi K, Yumoto H, Kido JI. 6-Shogaol inhibits advanced glycation end-products-induced IL-6 and ICAM-1 expression by regulating oxidative responses in human gingival fibroblasts. Molecules 2019; 24: 3705
• 10 Prasad S, Tyagi AK. Ginger and its constituents: Role in prevention and treatment of gastrointestinal cancer. Gastroenterol Res Pract 2015; 2015: 142979
• 11 Suekawa M, Ishige A, Yuasa K, Sudo K, Aburada M, Hosoya E. Pharmacological studies on ginger. I. Pharmacological actions of pungent constituents, (6)-gingerol and (6)-shogaol. J Pharmacobiodyn 1984; 7: 836-848
• 12 Gümüşay ÖA, Borazan AA, Ercal N, Demirkol O. Drying effects on the antioxidant properties of tomatoes and ginger. Food Chem 2015; 173: 156-162
• 13 Hao W, Chen Z, Yuan Q, Ma M, Gao C, Zhou Y, Zhou H, Wu X, Wu D, Farag MA, Wang S, Wang Y. Ginger polysaccharides relieve ulcerative colitis via maintaining intestinal barrier integrity and gut microbiota modulation. Int J Biol Macromol 2022; 219: 730-739
• 14 Wang Y, Wang S, Song R, Cai J, Xu J, Tang X, Li N. Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells. Int J Biol Macromol 2019; 123: 81-90
• 15 Aggarwal BB. Nuclear factor-κB: The enemy within. Cancer Cell 2004; 6: 203-208
• 16 Mantovani A. Inflammation by remote control. Nature 2005; 435: 752-753
• 17 Cao J, Tang D, Wang Y, Li X, Hong L, Sun C. Characteristics and immune-enhancing activity of pectic polysaccharides from sweet cherry (Prunus avium). Food Chem 2018; 254: 47-54
• 18 Wang P, Liao W, Fang J, Liu Q, Yao J, Hu M, Ding K. A glucan isolated from flowers of Lonicera japonica Thunb. inhibits aggregation and neurotoxicity of Aβ42. Carbohydr Polym 2014; 110: 142-147
• 19 Zhang S, Zhang C, Li M, Chen X, Ding K. Structural elucidation of a glucan from Crataegus pinnatifida and its bioactivity on intestinal bacteria strains. Int J Biol Macromol 2019; 128: 435-443
• 20 Lam JH, Baumgarth N. Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses. Nat Commun 2023; 14: 3979
• 21 Benalaya I, Alves G, Lopes J, Silva LR. A review of natural polysaccharides: Sources, characteristics, properties, food, and pharmaceutical applications. Int J Mol Sci 2024; 25: 1322
• 22 Ji X, Cheng Y, Tian J, Zhang S, Jing Y, Shi M. Structural characterization of polysaccharide from jujube (Ziziphus jujuba Mill.) fruit. Chem Biol Technol Agric 2021; 8: 54
• 23 Ji X, Guo J, Ding D, Gao J, Hao L, Guo X, Liu Y. Structural characterization and antioxidant activity of a novel high-molecular-weight polysaccharide from Ziziphus Jujuba cv. Muzao. J Food Meas Charact 2022; 16: 2191-2200
• 24 ShanChen. Khan BM, Cheong KL, Liu Y. Pumpkin polysaccharides: Purification, characterization and hypoglycemic potential. Int J Biol Macromol 2019; 139: 842-849
• 25 Yang X, Li A, Li X, Sun L, Guo Y. An overview of classifications, properties of food polysaccharides and their links to applications in improving food textures. Trends Food Sci Tech 2020; 102: 1-15
• 26 Li Y, Zhang X, Li Y, Yang P, Zhang Z, Wu H, Zhu L, Liu Y. Preparation methods, structural characteristics, and biological activity of polysaccharides from Salvia miltiorrhiza: A review. J Ethnopharmacol 2023; 305: 116090
• 27 Wang Y, Zhang H. Advances in the extraction, purification, structural-property relationships and bioactive molecular mechanism of Flammulina velutipes polysaccharides: A review. Int J Biol Macromol 2021; 167: 528-538
• 28 Lin X, Xiao B, Liu J, Cao M, Yang Z, Zhao L, Chen G. An acidic heteropolysaccharide rich in galactose and arabinose derived from ginger: Structure and dynamics. Food Biosci 2023; 56: 103127
• 29 Li Z, Du Z, Wang Y, Feng Y, Zhang R, Yan X. Chemical Modification, Characterization, and Activity Changes of Land Plant Polysaccharides: A Review. Polymers (Basel) 2022; 14: 4161
• 30 Sun L, Wang L, Zhou Y. Immunomodulation and antitumor activities of different-molecular-weight polysaccharides from Porphyridium cruentum . Carbohydr Polym 2012; 87: 1206-1210
• 31 Yin M, Zhang Y, Li H. Advances in research on immunoregulation of macrophages by plant polysaccharides. Front Immunol 2019; 10: 145
• 32 Coutinho-Wolino KS, Almeida PP, Mafra D, Stockler-Pinto MB. Bioactive compounds modulating Toll-like 4 receptor (TLR4)-mediated inflammation: pathways involved and future perspectives. Nutr Res 2022; 107: 96-116
• 33 Ji M, Sun L, Zhang M, Liu Y, Zhang Z, Wang P. RN0D, a galactoglucan from Panax notoginseng flower induces cancer cell death via PINK1/Parkin mitophagy. Carbohydr Polym 2024; 332: 121889
• 34 Wang P, Zhang L, Yao J, Shi Y, Li P, Ding K. An arabinogalactan from flowers of Panax notoginseng inhibits angiogenesis by BMP2/Smad/Id1 signaling. Carbohydr Polym 2015; 121: 328-335
• 35 Cong Q, Chen H, Liao W, Xiao F, Wang P, Qin Y, Dong Q, Ding K. Structural characterization and effect on anti-angiogenic activity of a fucoidan from Sargassum fusiforme . Carbohydr Polym 2016; 136: 899-907