Planta Med 2024; 90(11): 885-895
DOI: 10.1055/a-2341-6175
Biological and Pharmacological Activity
Original Papers

5(S)-5-Carboxystrictosidine from the Root of Mappianthus iodoides Ameliorates H2O2-induced Apoptosis in H9c2 Cardiomyocytes via PI3K/AKT and ERK Pathways

Authors

  • Ying Han

    1   Department of Physiology, College of Chinese Medicine and Life Science, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
    5   Key Laboratory of Psychology of TCM and Brain Science, Jiangxi Administration of traditional Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
  • Junli Xi

    1   Department of Physiology, College of Chinese Medicine and Life Science, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
  • Puzhao Zhang

    2   Key Laboratory of Innovation Drug and Efficient Energy-saving Pharmaceutical Equipment, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
  • Ming Gong

    1   Department of Physiology, College of Chinese Medicine and Life Science, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
  • Tao Luo

    3   Blood Purification Center of the First Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province, China
  • Feng Shao

    2   Key Laboratory of Innovation Drug and Efficient Energy-saving Pharmaceutical Equipment, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
  • Yongxin Li

    1   Department of Physiology, College of Chinese Medicine and Life Science, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
  • Lingyun Zhong

    4   College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
  • Hexiu Quan

    1   Department of Physiology, College of Chinese Medicine and Life Science, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China

Supported by: Top Discipline of Jiangxi Province,Discipline of Chinese and Western Integrative Medicine, Jiangxi University of Chinese Medicine zxyylxk20220103 Supported by: The Graduate Student Innovation special Fund Project of Jiangxi Province YC2021-S485 Supported by: Inherited and Innovative Group of Processing Technique of Traditional Chinese Medicine CXTD22003 Supported by: Science and Technology Project of Jiangxi Provincial Health Commission 202211410 Supported by: The Science and Technology Research Project of Jiangxi Provincial Department of Education, China GJJ2200958 Supported by: The Science and Technology Research Project of Jiangxi Provincial Department of Education, China GJJ211204 Supported by: Innovation and Entrepreneurship training program for college students in Jiangxi Province X202310412181 Supported by: Science and technology project of Jiangxi Provincial Administration of Traditional Chinese Medicine 2023B1182
Preview

Abstract

5(S)-5-carboxystrictosidine (5-CS) is a compound found in the root of Mappianthus iodoides, a traditional Chinese medicine used for the treatment of coronary artery disease. The aim of the present study was to investigate the protective effect of 5-CS against oxidative stress-induced apoptosis in H9c2 cardiomyocytes and the underlying mechanisms. 5-CS pretreatment significantly protected against H2O2-induced cell death, LDH leakage, and malondialdehyde (MDA) production, which are indicators for oxidative stress injury. 5-CS also enhanced the activity of SOD and CAT. In addition, 5-CS pretreatment significantly inhibited H2O2-induced apoptosis, as determined by flow cytometer, suppressed the activity of caspase-3 and caspase-9, and attenuated the activation of cleaved caspase-3 and caspase-9. 5-CS also increased Akt and ERK activation altered by H2O2 using Western blot analysis. The PI3K-specific inhibitor LY294002 abolished 5-CS-induced Akt activation. The ERK-specific inhibitor PD98059 abolished 5-CS-induced ERK activation. Both LY294002 and PD98059 attenuated the protective effect of 5-CS on H9c2 cardiomyocytes against H2O2-induced apoptosis and cell death. Taken together, these results demonstrate that 5-CS prevents H2O2-induced oxidative stress injury in H9c2 cells by enhancing the activity of the endogenous antioxidant enzymes, inhibiting apoptosis, and modulating PI3K/Akt and ERK signaling pathways.

Supporting Information



Publication History

Received: 14 November 2023

Accepted after revision: 10 June 2024

Accepted Manuscript online:
10 June 2024

Article published online:
19 July 2024

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