Planta Med 2022; 88(14): 1311-1324
DOI: 10.1055/a-1721-4971
Biological and Pharmacological Activity
Original Papers

Raw and Wine Processed Schisandra chinensis Regulate NREM-Sleep and Alleviate Cardiovascular Dysfunction Associated with Insomnia by Modulating HPA Axis

Dan Su
1   Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, Nanchang, China
,
Jian Luo
1   Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, Nanchang, China
,
Junqi Ge
1   Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, Nanchang, China
,
Yali Liu
1   Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, Nanchang, China
,
Chen Jin
1   Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, Nanchang, China
,
Pengfei Xu
1   Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, Nanchang, China
,
Ruowen Zhang
2   Shenzhen Honsan Health Industry Group, Shenzhen, China
,
Genhua Zhu
1   Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, Nanchang, China
,
Ming Yang
1   Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, Nanchang, China
,
Zhifu Ai
1   Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, Nanchang, China
,
Yonggui Song
1   Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, Nanchang, China
› Author Affiliations

Supported by: Establishment project of Jiangxi science and technology innovation platform 20212BCD42009 Supported by: Science and technology project of Jiangxi Administration of traditional Chinese Medicine 2020Z003 Supported by: Science and technology project of Jiangxi Administration of traditional Chinese Medicine 2021Z011 Supported by: Jiangxi Provincial Natural Science Foundation 20202BABL206142 Supported by: Jiangxi Provincial Natural Science Foundation 20212BAB206012 Supported by: National Natural Science Foundation of China 81860702 Supported by: National Natural Science Foundation of China 82060808 Supported by: National Natural Science Foundation of China 82074014 Supported by: Jiangxi University of Chinese Medicine Graduate Innovation Fund S202110412001 Supported by: Jiangxi University of Chinese Medicine Graduate Innovation Fund JZYC21S32 Supported by: Science and Technology Key Project of Jiangxi Provincial Department of Education GJJ201206
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Abstract

Clinical studies have shown that insomnia and anxiety are usually accompanied by cardiovascular dysfunction. In traditional Chinese medicine, Schisandra chinensis (SC) and wine processed Schisandra chinensis (WSC) are mainly used for the treatment of dysphoria, palpitation and insomnia. However, little attention was paid to its mechanism. In this study, we monitored the effect of SC and WSC on the nervous system and cardiovascular system of free-moving rats in the real-time. Our results show that SC and WSC can alleviate cardiovascular dysfunction while promoting sleep, and we further explored their potential mechanisms. HPLC-QTOF-MS was used for the quality control of chemical components in SC and WSC. Data sciences international (DSI) physiological telemetry system was applied to collect the electroencephalogram (EEG), electrocardiogram (ECG) and other parameters of free-moving rats to understand the effects of long-term intake of SC and WSC on rats. The content of Cortisol (CORT), neurotransmitters and amino acids in rat pituitary and hypothalamus were analyzed by UPLC-MS to determine the activity of HPA axis. The expression of melatonin receptor MT1 was analyzed by immunofluorescence technique. Our results suggested that SC and WSC may play the role of promoting sleep by increasing the expression level of melatonin receptor MT1 in hypothalamus, and modulate the activity of HPA axis by regulating the levels of the related neurotransmitters and amino acid, so as to improve the abnormal cardiovascular system of rats. This study may provide theoretical support for explicating the advantages of SC and other phytomedicines in the treatment of insomnia.

Supporting Information



Publication History

Received: 21 September 2021

Accepted after revision: 15 December 2021

Accepted Manuscript online:
15 December 2021

Article published online:
28 February 2022

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