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Int J Sports Med 2022; 43(07): 632-641
DOI: 10.1055/a-1717-1693
Training & Testing

Effect of 12-wk Training in Ovariectomised Rats on PGC-1α, NRF-1 and Energy Substrates

Taciane Maria Melges Pejon 
1   Laboratory of Endocrine Physiology and Physical Exercise, Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil
,
Vinicius Silva Faria
1   Laboratory of Endocrine Physiology and Physical Exercise, Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil
,
Claudio Alexandre Gobatto
2   Laboratory of Applied Sport Physiology, Department of Sport Sciences, School of Applied Sciences, State University of Campinas, Limeira, São Paulo, Brazil
,
Fúlvia Barros Manchado-Gobatto 
2   Laboratory of Applied Sport Physiology, Department of Sport Sciences, School of Applied Sciences, State University of Campinas, Limeira, São Paulo, Brazil
,
Pedro Paulo Menezes Scariot
2   Laboratory of Applied Sport Physiology, Department of Sport Sciences, School of Applied Sciences, State University of Campinas, Limeira, São Paulo, Brazil
,
Anabelle Silva Cornachione
3   Muscle Physiology and Biophysics Laboratory, Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil
,
Wladimir Rafael Beck
3   Muscle Physiology and Biophysics Laboratory, Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil
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Abstract

Metabolic diseases are associated with hypoestrogenism owing to their lower energy expenditure and consequent imbalance. Physical training promotes energy expenditure through PGC-1α and NRF-1, which are muscle proteins of the oxidative metabolism. However, the influence of physical training on protein expression in individuals with hypoestrogenism remains uncertain. Thus, the aim of this study is to determine the effect of 12 weeks of moderate-intensity swimming training on the muscle expression of PGC-1α, NRF-1, glycogen and triglyceride in ovariectomised rats. OVX and OVX+TR rats were subjected to ovariectomy. The trained animals swam for 30 minutes, 5 days/week, at 80% of the critical load intensity. Soleus was collected to quantify PGC-1α and NRF-1 expressions, while gastrocnemius and gluteus maximus were collected to measure glycogen and triglyceride. Blood glucose was also evaluated. Whereas ovariectomy decreased PGC-1α expression (p<0.05) without altering NRF-1 (p=0.48), physical training increased PGC-1α (p<0.01) and NRF-1 (p<0.05). Ovariectomy reduced glycogen (p<0.05) and triglyceride (p<0.05), whereas physical training increased glycogen (p<0.05) but did not change triglyceride (p=0.06). Ovariectomy increased blood glucose (p<0.01), while physical training reduced it (p<0.01). In summary, 12 weeks of individualized and moderate-intensity training were capable of preventing muscle metabolic consequences caused by ovariectomy.

Key words

oestrogen deficit - hypoestrogenism - moderate intensity training - metabolism - female rats - energetic imbalance


Publication History

Received: 18 June 2021

Accepted: 15 November 2021

Article published online:
18 February 2022

© 2021. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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