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Planta Med 2022; 88(09/10): 721-728
DOI: 10.1055/a-1712-8209
Biological and Pharmacological Activity
Original Papers

Anti-inflammatory Constituents from Caulis Trachelospermi [ # ]

Hongzhi Song‡
1   Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Guilin Medical University, Guilin, China
2   School of Medicine, Shanghai University, Shanghai, China
,
Jinni Tan‡
1   Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Guilin Medical University, Guilin, China
,
Ruijing Ma
1   Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Guilin Medical University, Guilin, China
,
Edward J Kennelly
3   Department of Biological Sciences, Lehman College, City University of New York, Bronx, New York, USA
4   Ph.D. Program in Biology, The Graduate Center, City University of New York, New York, USA
,
Qingang Tan 
1   Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Guilin Medical University, Guilin, China
5   Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences; Guilin, China
6   State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Guilin, China
› Author AffiliationsSupported by: National Natural Science Foundation of China 82060783
Supported by: State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University) CMEMR2018-B03
Supported by: Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization FPRU2018-2
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Abstract

Caulis Trachelospermi, the stems with leaves of Trachelospermum jasminoides, is a well-known herbal drug of the Apocynaceae family recorded in the Chinese pharmacopeia and used for the treatment of inflammation-related diseases by ethnic minorities of China. The mechanism of anti-inflammatory activity and responsible constituents of T. jasminoides have not been well elucidated in previous studies. Preliminary investigation showed that both the water and the ethyl ester extracts of T. jasminoides exhibited potent inhibitory activity on nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated murine macrophages. Phytochemical investigation on these extracts afforded 23 compounds, including three new compounds (1 –3) identified on the basis of spectroscopic and mass spectrometric data. Anti-inflammatory bioassay showed that compounds 17, 18, 22, and 23 inhibited significantly the production of NO in a concentration-dependent manner. Further studies indicated that compound 23 inhibited significantly TNF-α and IL-6 produced by LPS-stimulated RAW 264.7 cells with good selectivity, as well as protein expression of iNOS in RAW 264.7 cells. These chemical constituents may contribute to the anti-inflammatory potential of T. jasminoides.

Key words

Trachelospermum jasminoides - Apocynaceae - triterpenoid glycosides - NO production - tumor necrosis factor-α - interleukin-6

# Dedicated to Professor Dr. Douglas A. Kinghorn on the occasion of his 75th birthday.


Supporting Information

    The detailed extraction and isolation procedure, NMR and HRESIMS data of the new compounds (1 – 3) (Figs. 1S – 28S), the determination of hydrolysis product of 1 (Fig. 29S), the base peak integration (BPI) chromatograms of the plant total methanol extract (Fig. 30S), chemical structures of all the isolated compounds (Fig. 31S), and the relative viability of RAW 264.7 cells treated with different compounds (Table 1S) are included in Supporting Information.

  • Supporting Information


Publication History

Received: 12 September 2021

Accepted after revision: 25 November 2021

Article published online:
28 December 2021

© 2021. Thieme. All rights reserved.

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