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Planta Med 2021; 87(14): 1152-1166
DOI: 10.1055/a-1647-2930
Natural Product Chemistry and Analytical Studies
Original Papers

HPTLC Fingerprint Authentication of Selected Sideritis spp. Using a Pharmacognostic Approach[ # ]

Lisa-Anna-Maria Pihan
1   Zurich University of Applied Sciences, Institute of Chemistry and Biotechnology, Natural Products and Phytopharmacy Research Group, Wädenswil, Switzerland
2   Technical University Dresden, Faculty of Biology, Dresden, Germany
,
Samuel Peter
1   Zurich University of Applied Sciences, Institute of Chemistry and Biotechnology, Natural Products and Phytopharmacy Research Group, Wädenswil, Switzerland
,
Günter Vollmer
2   Technical University Dresden, Faculty of Biology, Dresden, Germany
,
Beat Meier
1   Zurich University of Applied Sciences, Institute of Chemistry and Biotechnology, Natural Products and Phytopharmacy Research Group, Wädenswil, Switzerland
,
Evelyn Wolfram
1   Zurich University of Applied Sciences, Institute of Chemistry and Biotechnology, Natural Products and Phytopharmacy Research Group, Wädenswil, Switzerland
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Abstract

The genus Sideritis (Lamiaceae) comprises around 150 species, of which many are popular herbal remedies in Mediterranean folk medicine. Already mentioned by Dioscorides and Theophrastus, the “ironwort” or “Greek mountain tea” has been receiving increased attention in recent years. A European Union herbal monograph and assessment report (HMPC) has been issued, covering the species Sideritis scardica, S. clandestina, S. raeseri, and S. syriaca. This study presents results of a first pharmacognostic examination of the botanical and phytochemical differences among and between these emerging commercial species, and other, less studied species. An HPTLC method is proposed for normal phase separation of the species; this means applying two mobile phases on silica plates and subsequent derivatization with natural product reagent (NP/PEG) for visualization of phenolic compounds and anisaldehyde for a broader detection. With the help of selected reference compounds, a system suitability test was established for proper chromatographic separation. The method was applied to specimens from botanical gardens and commercial raw material in order to test its suitability for differentiation and authentication. The HPTLC analysis also includes, for the first time, S. hyssopifolia and other less used Sideritis species. The results might enable the development of a validated phytochemical fingerprint authentication procedure for quality assurance of Sideritis herba.

Key words

HPTLC - authentication - Lamiaceae - Sideritis spp. - Sideritis scardica - Sideritis raeseri - Sideritis hyssopifolia

# Dedicated to Professor Dr. Otto Sticher on the occasion of his 85th birthday.


Supporting Information

    Photographs for macroscopic morphological comparison (Fig. 1S), quantification of total polyphenols and flavonoids in the extracts (Fig. 2S), fingerprints of extracts with and without glandular hairs (Fig. 3S), HPTLC chromatograms for assessing elution and color of reference substances under different conditions (Fig. 4S–6S), details on the plant material (Table 1S), and the different mobile phases and derivatization conditions (Table 2S) are provided as supporting information. Table 3S provides an overview of the tested derivatization reagents and how they were prepared.

  • Supporting Information


Publication History

Received: 12 April 2021

Accepted after revision: 24 August 2021

Article published online:
15 October 2021

© 2021. Thieme. All rights reserved.

Georg Thieme Verlag KG
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